Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil and.
J Enzyme Inhib Med Chem. 2014 Feb;29(1):12-7. doi: 10.3109/14756366.2012.743537. Epub 2013 Jan 17.
Abstract Lippia alba (Miller) N.E. Brown is an aromatic plant known locally as "Erva-cidreira-do-campo" that has great importance in Brazilian folk medicine. The aim of our study was to evaluate the antidermatophytic potential of linalool-rich essential oil (EO) from L. alba and analyze the ability of this EO to inhibit peptidase and keratinase activities, which are important virulence factors in dermatophytes. The minimum inhibitory concentrations (MICs) of L. alba EO were 39, 156 and 312 µg/mL against Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum, respectively. To evaluate the influence of L. alba EO on the proteolytic and keratinolytic activities of these dermatophytes, specific inhibitory assays were performed. The results indicated that linalool-rich EO from L. alba inhibited the activity of proteases and keratinases secreted from dermatophytes, and this inhibition could be a possible mechanism of action against dermatophytes. Due to the effective antidermatophytic activity of L. alba EO, further experiments should be performed to explore the potential of this linalool-rich EO as an alternative antifungal therapy.
摘要 香根草（Miller）N.E.布朗是一种芳香植物，在巴西民间医学中被称为“Erva-cidreira-do-campo”。本研究旨在评估富含里哪醇的香根草精油（EO）的抗皮肤真菌潜力，并分析这种 EO 抑制肽酶和角蛋白酶活性的能力，这些酶是皮肤真菌中的重要毒力因子。香根草 EO 对红色毛癣菌、絮状表皮癣菌和石膏样小孢子菌的最小抑菌浓度（MIC）分别为 39、156 和 312μg/mL。为了评估香根草 EO 对这些皮肤真菌蛋白酶和角蛋白酶活性的影响，进行了特定的抑制试验。结果表明，香根草富含里哪醇的 EO 抑制了皮肤真菌分泌的蛋白酶和角蛋白酶的活性，这种抑制可能是其抗皮肤真菌的作用机制之一。由于香根草 EO 具有有效的抗皮肤真菌活性，因此应进一步进行实验，以探索这种富含里哪醇的 EO 作为替代抗真菌治疗的潜力。
