Mert Tufan, Ocal Isıl, Guzel A Irfan, Gunay Ismail
Department of Biophysics, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
Electromagn Biol Med. 2013 Sep;32(3):342-54. doi: 10.3109/15368378.2012.721846. Epub 2013 Jan 16.
Several studies have reported that pulsed magnetic fields (PMFs) can be a choice of therapy for diabetic peripheral neuropathy. However, the exact underlying mechanism of PMF is still not known. The purpose of this study was, therefore, to investigate the effects of clodronate encapsulated with liposome, a specific agent depleting macrophage, on PMF-treated streptozotocin-induced type I diabetic rats with peripheral neuropathy. Effects of PMF, liposome-encapsulated clodronate (LEC) or their combined treatments were investigated in diabetic rats by measuring the thermal latencies, mechanical thresholds, whole blood glucose levels, serum insulin level, and body mass. In diabetic rats, PMF exhibited a decrease in the blood glucose levels but did not change the serum insulin level. Both mechanical thresholds and thermal latencies of diabetic rats enhanced throughout the PMF treatment. During the PMF treatment, the administration of LEC suppressed the PMF-induced decrease in blood glucose level, PMF-induced increase in mechanical threshold and thermal latencies in diabetic animals. In addition, PMF reduced the LEC-induced increase in insulin levels of diabetic rats. Findings demonstrated that although effects of both PMF alone and LEC alone on diabetic animals are mostly positive, LEC may remove the therapeutic efficacies of PMF in combined treatment.
多项研究报告称，脉冲磁场（PMF）可作为治疗糖尿病周围神经病变的一种选择。然而，PMF确切的潜在机制仍不清楚。因此，本研究的目的是探讨用脂质体包裹的氯膦酸盐（一种特异性巨噬细胞耗竭剂）对经PMF治疗的链脲佐菌素诱导的I型糖尿病周围神经病变大鼠的影响。通过测量热潜伏期、机械阈值、全血糖水平、血清胰岛素水平和体重，研究了PMF、脂质体包裹的氯膦酸盐（LEC）或它们的联合治疗对糖尿病大鼠的影响。在糖尿病大鼠中，PMF使血糖水平降低，但血清胰岛素水平未改变。在整个PMF治疗过程中，糖尿病大鼠的机械阈值和热潜伏期均增强。在PMF治疗期间，给予LEC可抑制PMF诱导的糖尿病动物血糖水平降低、PMF诱导的机械阈值和热潜伏期增加。此外，PMF降低了LEC诱导的糖尿病大鼠胰岛素水平升高。研究结果表明，虽然单独使用PMF和单独使用LEC对糖尿病动物的影响大多是积极的，但在联合治疗中，LEC可能会消除PMF的治疗效果。
