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一种与蜜蜂螺旋体原纤维相关的57千道尔顿蛋白质会发生可逆磷酸化。

A 57-kilodalton protein associated with Spiroplasma melliferum fibrils undergoes reversible phosphorylation.

作者信息

Platt M W, Reizer J, Rottem S

机构信息

Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

出版信息

J Bacteriol. 1990 May;172(5):2808-11. doi: 10.1128/jb.172.5.2808-2811.1990.

DOI:10.1128/jb.172.5.2808-2811.1990
PMID:2332410
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208935/
Abstract

Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in the cytosolic fraction and, to a lesser extent, associated with cell membranes and was detected in the Triton X-100-insoluble fraction that contained fibrils.

摘要

在用[γ-32P]ATP孵育的蜂蜜螺旋体BC3细胞裂解物中观察到一种主要的57千道尔顿蛋白质底物的磷酸化。从磷酸化蛋白质的酸水解产物中仅分离出丝氨酸磷酸盐。发现57千道尔顿蛋白质底物在很大程度上存在于胞质部分，在较小程度上与细胞膜相关，并在含有原纤维的Triton X-100不溶性部分中检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaab/208935/8951d6932681/jbacter00119-0624-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaab/208935/ae452223c0cf/jbacter00119-0623-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaab/208935/8951d6932681/jbacter00119-0624-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaab/208935/ae452223c0cf/jbacter00119-0623-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eaab/208935/8951d6932681/jbacter00119-0624-a.jpg

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本文引用的文献

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Purification and preliminary characterization of Spiroplasma fibrils.螺原体纤丝的纯化及初步特性分析
J Bacteriol. 1980 May;142(2):694-700. doi: 10.1128/jb.142.2.694-700.1980.
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Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents.用非离子去污剂处理的粘附性肺炎支原体细胞中的丝状结构。
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Yale J Biol Med. 1983 Sep-Dec;56(5-6):419-23.
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ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes.化脓性链球菌磷酸转移酶系统的磷酸载体蛋白HPr中丝氨酰残基的ATP依赖性蛋白激酶催化磷酸化作用
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Unusual fibrils from the spirochete-like sex ratio organism.来自类螺旋体性别比例生物的异常纤丝。
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Binding of phosphorylated and dephosphorylated heavy meromyosin to F-actin.磷酸化和去磷酸化重酶解肌球蛋白与F-肌动蛋白的结合。
FEBS Lett. 1987 Jan 5;210(2):177-80. doi: 10.1016/0014-5793(87)81332-7.
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Protein phosphorylation in bacterial chemotaxis.细菌趋化作用中的蛋白质磷酸化
Cell. 1988 Apr 8;53(1):1-2. doi: 10.1016/0092-8674(88)90478-3.
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Characterization of membrane components of the flask-shaped mycoplasma Mycoplasma mobile.烧瓶状支原体“运动支原体”膜成分的特性分析
J Gen Microbiol. 1988 Aug;134(8):2385-92. doi: 10.1099/00221287-134-8-2385.
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Phosvitin kinase activity in Acholeplasma axanthum.无黄无胆甾原体中的卵黄高磷蛋白激酶活性。
FEBS Lett. 1988 Dec 19;242(1):97-100. doi: 10.1016/0014-5793(88)80993-1.