吸烟者中慢性阻塞性肺疾病患者与非患者肺组织沉默调节蛋白表达的不同模式。

Different patterns of lung sirtuin expression in smokers with and without chronic obstructive pulmonary disease.

机构信息

Department of Pathology, Faculty of Medicine, University of Latvia, Sarlotes 1A, 1001 Riga, Latvia.

出版信息

Medicina (Kaunas). 2012;48(10):552-7.

Abstract

BACKGROUND AND OBJECTIVE

Chronic obstructive pulmonary disease is characterized by persistent and modified inflammatory responses in lung. Human sirtuin, an antiaging and antiinflammatory protein, is a metabolic NAD(+)-dependent protein/histone deacetylase that regulates proinflammatory mediators by deacetylating histone and nonhistone proteins. The aim of our study was to compare the expression of sirtuin in large and small airways in nonsmokers, asymptomatic smokers, and smokers with chronic obstructive pulmonary disease.

MATERIAL AND METHODS

A total of 12 nonsmokers, 14 asymptomatic smokers, and 12 smokers with moderate chronic obstructive pulmonary disease were enrolled into the study. Immunohistochemical and Western blot methods were used to analyze sirtuin expression in the airways.

RESULTS

The obtained results showed the nonuniform sirtuin expression throughout the bronchial tree. Smokers both with and without chronic obstructive pulmonary disease had decreased sirtuin expression in large airways. However, in small airways, sirtuin expression was decreased only in patients with chronic obstructive pulmonary disease. In addition, a correlation between airflow limitation, smoked pack-years and the number of sirtuin-positive cells in airways was found.

CONCLUSIONS

Smoking is characterized by suppressed sirtuin expression in large airways, whereas chronic obstructive pulmonary disease is characterized by more severe suppression of sirtuin expression both in large and small airways.

摘要

背景与目的

慢性阻塞性肺疾病的特征是肺部持续和改变的炎症反应。人类的 Sirtuin 是一种抗衰老和抗炎蛋白，是一种代谢 NAD(+)依赖性蛋白/组蛋白去乙酰化酶，通过去乙酰化组蛋白和非组蛋白蛋白来调节促炎介质。我们研究的目的是比较非吸烟者、无症状吸烟者和慢性阻塞性肺疾病吸烟者大、小气道中 Sirtuin 的表达。