Department of Ophthalmology, Hebei Medical University Fourth Hospital, Shijiazhuang, Hebei 050011, China.
Chin Med J (Engl). 2013 Jan;126(2):340-4.
Retinocytoma (RB) is a very common intraocular malignant tumor during infancy. Chemotherapy has gradually been used as the first-line treatment for intraocular RB in recent years. In this study, Livin and PTEN expressions were observed in the RB tissue, along with the growth-inhibiting and apoptosis-induced effects of topotecan (TPT) on RB HXO-Rb44 cell strain. This study aimed to investigate the antigrowth effects of TPT on RB cell strain HXO-Rb44.
Max-Vision(TM) rapid immunohistochemistry was adopted to detect Livin and PTEN expressions in the normal retina and in RB, and their relationship with RB clinicopathologic features was analyzed. Human RB cell strain HXO-Rb44 was cultivated and passaged. MTT method was used to measure the survival rates of HXO-Rb44 cell strains under various TPT concentrations. IC50 values were calculated. Flow cytometry was used to detect the effects of various TPT concentrations on HXO-Rb44 cell apoptosis. Western blotting was used to detect the differences of Livin and PTEN protein expressions during cell apoptosis.
The positive expressions of Livin and PTEN in the RB group were obviously different from those in the normal control group. In RB tissue, Livin expression was relevant to PTEN expression. TPT could significantly induce the occurrence of cell apoptosis and had a dependent relationship with drug concentration. Livin and PTEN expression levels varied with the extension of the effect time of TPT based on Western blotting analysis.
Livin and PTEN have high and low expression levels in the RB tissue, respectively. Both of them have key roles in RB occurrence and development. TPT could induce human RB cell strain HXO-Rb44 cell apoptosis, and its mechanism is associated with the inhibition of Livin and PTEN expressions.
视网膜母细胞瘤（RB）是婴幼儿期常见的眼内恶性肿瘤。近年来，化疗逐渐成为眼内 RB 的一线治疗方法。本研究观察了 Livin 和 PTEN 在 RB 组织中的表达，并观察了拓扑替康（TPT）对 RB HXO-Rb44 细胞株的生长抑制和凋亡诱导作用。本研究旨在探讨 TPT 对 RB 细胞株 HXO-Rb44 的抗增殖作用。
采用 Max-Vision（TM）快速免疫组化法检测正常视网膜和 RB 中 Livin 和 PTEN 的表达，并分析其与 RB 临床病理特征的关系。培养并传代人 RB 细胞株 HXO-Rb44。MTT 法检测不同 TPT 浓度下 HXO-Rb44 细胞株的存活率，计算 IC50 值。流式细胞术检测不同 TPT 浓度对 HXO-Rb44 细胞凋亡的影响。Western blot 法检测细胞凋亡过程中 Livin 和 PTEN 蛋白表达的差异。
RB 组 Livin 和 PTEN 的阳性表达明显不同于正常对照组。在 RB 组织中，Livin 表达与 PTEN 表达相关。TPT 能显著诱导细胞凋亡，并与药物浓度呈依赖性关系。Western blot 分析显示，Livin 和 PTEN 表达水平随 TPT 作用时间的延长而变化。
RB 组织中 Livin 和 PTEN 呈高低表达。两者均在 RB 的发生发展中起关键作用。TPT 可诱导人 RB 细胞株 HXO-Rb44 细胞凋亡，其机制与抑制 Livin 和 PTEN 表达有关。
