Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei, China.
J Xray Sci Technol. 2012;20(4):459-68. doi: 10.3233/XST-2012-00352.
The combined system of micro-CT and fluorescence molecular tomography (FMT) offers a new tool to provide anatomical and functional information of small animals in a single study. To take advantages of the combined system, a data preprocessing method is proposed to extract the valid data for FMT reconstruction algorithms using a priori information provided by CT. The boundary information of the animal and animal holder is extracted from reconstructed CT volume data. A ray tracing method is used to trace the path of the excitation beam, calculate the locations and directions of the optional sources and determine whether the optional sources are valid. To accurately calculate the projections of the detectors on optical images and judge their validity, a combination of perspective projection and inverse ray tracing method are adopted to offer optimal performance. The imaging performance of the combined system with the presented method is validated through experimental rat imaging.
微计算机断层扫描和荧光分子断层成像（FMT）的联合系统为提供小动物的解剖学和功能信息提供了一种新的工具，可在单次研究中实现。为了利用联合系统的优势，提出了一种数据预处理方法，利用 CT 提供的先验信息，从重建的 CT 体数据中提取用于 FMT 重建算法的有效数据。从重建的 CT 体数据中提取动物和动物支架的边界信息。使用光线追踪方法来追踪激发光束的路径，计算可选源的位置和方向，并确定可选源是否有效。为了准确计算探测器在光学图像上的投影并判断其有效性，采用透视投影和反向光线追踪方法的组合来提供最佳性能。通过实验大鼠成像验证了所提出方法的联合系统的成像性能。
