Faculty of Pharmacy, Meijo University, Tempaku-ku, Nagoya, Aichi, Japan.
Inflamm Res. 2013 Mar;62(3):333-41. doi: 10.1007/s00011-012-0586-8. Epub 2013 Jan 17.
Mast cells play a central role in allergic and chronic inflammation. Extracts from Clausena lansium (Lour.) Skeels (Rutaceae) possess many pharmacological effects including anti-inflammatory, anti-oxidant, anti-cancer, and anti-trichomonal activities. In addition, the leaves and fruit are used in Chinese folk medicine. We have isolated and identified four known cinnamamides from this plant: lansiumamide C, lansamide I, lansiumamide B, and SB-204900. However, the biological activities of these compounds are not yet understood. The purpose of this paper is to clarify the pharmacological effects of these compounds on mast cells.
We measured inflammatory molecules in A23187-stimulated rat basophilic leukemia cells (RBL-2H3) treated with these compounds using HPLC, ELISA, and immunoblotting methods. In addition, some signaling molecules were investigated by immunoblotting.
Lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release. Furthermore, lansiumamide B- and SB-204900-treated cells also reduced the protein and/or mRNA levels of TNF-α. SB-204900 markedly suppressed the phosphorylation of p38 MAPK.
Our findings suggest that lansiumamide B and SB-204900 attenuate mast-cell-induced inflammation.
肥大细胞在过敏和慢性炎症中起核心作用。罗望子（Clausena lansium (Lour.) Skeels）的提取物具有许多药理作用，包括抗炎、抗氧化、抗癌和抗滴虫活性。此外，其叶和果实被用于中国民间医学。我们已经从这种植物中分离并鉴定出四种已知的肉桂酰胺： lansiumamide C、lansamide I、lansiumamide B 和 SB-204900。然而，这些化合物的生物学活性尚不清楚。本文旨在阐明这些化合物对肥大细胞的药理作用。
我们使用 HPLC、ELISA 和免疫印迹法测量了这些化合物处理 A23187 刺激的大鼠嗜碱性白血病细胞（RBL-2H3）后炎症分子的变化。此外，还通过免疫印迹法研究了一些信号分子。
Lansamide I、lansiumamide B 和 SB-204900 显著降低了组胺的释放。此外，lansiumamide B 和 SB-204900 处理的细胞还降低了 TNF-α的蛋白和/或 mRNA 水平。SB-204900 明显抑制了 p38 MAPK 的磷酸化。
我们的研究结果表明，lansiumamide B 和 SB-204900 可减轻肥大细胞诱导的炎症。
