Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, South Korea.
Inflammation. 2012 Oct;35(5):1723-31. doi: 10.1007/s10753-012-9490-1.
Type I allergy is characterized by the release of granule-associated mediators, lipid-derived substances, cytokines, and chemokines by activated mast cells. To evaluate the anti-allergic effects of macelignan isolated from Myristica fragrans Houtt., we determined its ability to inhibit calcium (Ca(2+)) influx, degranulation, and inflammatory mediator production in RBL-2 H3 cells stimulated with A23187 and phorbol 12-myristate 13-acetate. Macelignan inhibited Ca(2+) influx and the secretion of β-hexosaminidase, histamine, prostaglandin E(2), and leukotriene C(4); decreased mRNA levels of cyclooxygenase-2, 5-lipoxygenase, interleukin-4 (IL-4), IL-13, and tumor necrosis factor-α; and attenuated phosphorylation of Akt and the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. These results indicate the potential of macelignan as a type I allergy treatment.
I 型过敏反应的特征是激活的肥大细胞释放颗粒相关介质、脂质衍生物质、细胞因子和趋化因子。为了评估从肉豆蔻（Myristica fragrans Houtt.）中分离出的马卡利尼干（macelignan）的抗过敏作用，我们测定了其抑制 A23187 和佛波醇 12-肉豆蔻酸 13-醋酸盐刺激的 RBL-2 H3 细胞中钙（Ca(2+)）内流、脱颗粒和炎症介质产生的能力。马卡利尼干抑制 Ca(2+)内流和β-己糖胺酶、组胺、前列腺素 E(2)和白三烯 C(4)的分泌；降低环加氧酶-2、5-脂氧合酶、白细胞介素-4 (IL-4)、白细胞介素-13 和肿瘤坏死因子-α的 mRNA 水平；并减弱 Akt 和丝裂原激活的蛋白激酶细胞外信号调节激酶、p38 和 c-Jun N-末端激酶的磷酸化。这些结果表明马卡利尼干作为 I 型过敏治疗的潜力。
