Toh Benjamin, Nardin Alessandra, Dai Xilei, Keeble Jo, Chew Valerie, Abastado Jean-Pierre
Singapore Immunology Network, BMSI, A-STAR, Singapore, Singapore.
Methods Mol Biol. 2013;961:261-77. doi: 10.1007/978-1-62703-227-8_17.
Tumor-infiltrating immune cells have long been thought to affect tumor growth. In recent years, large retrospective studies have shown that the nature and polarization of the immune cells found within the tumor microenvironment impact not only the growth of the primary tumor, but also disease progression and patient survival. This has triggered considerable interest for an in depth analysis of the tumoral immune microenvironment and has created a need for standardized methods to characterize tumor-infiltrating immune cells. Here, we describe three approaches that can be used in mouse and human melanoma tumors.
长期以来，人们一直认为肿瘤浸润免疫细胞会影响肿瘤生长。近年来，大型回顾性研究表明，肿瘤微环境中发现的免疫细胞的性质和极化不仅影响原发性肿瘤的生长，还影响疾病进展和患者生存。这引发了对深入分析肿瘤免疫微环境的极大兴趣，并产生了对标准化方法来表征肿瘤浸润免疫细胞的需求。在此，我们描述了三种可用于小鼠和人类黑色素瘤肿瘤的方法。
