Malekpour Monireh, Etebari Atefe, Hezarosi Mahsa, Anissian Ali, Karimi Forouzan
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Veterinary, Abhar Branch, Islamic Azad University, Abhar, Iran.
Iran J Pharm Res. 2022 Sep 30;21(1):e130881. doi: 10.5812/ijpr-130881. eCollection 2022 Dec.
Irritant contact dermatitis is a common inflammatory skin disease characterized by skin barrier dysfunction, eczematous dermatitis, and chronic itching. This disease severely affects the quality of life. Considering that the current treatment approaches are not ideal, more extensive research is needed to develop new treatments. Mainly, a mouse model is needed to investigate the effectiveness of new drugs to treat this disease.
This study was conducted to create a mouse model of irritant contact dermatitis.
In the current study, we used BALB/c female mice to prepare a mouse model of irritant contact dermatitis. To induce irritant contact dermatitis, we used a dinitrochlorobenzene mixture with acetone/olive oil as an irritant. After 10 days of application, the mouse skin tissue was isolated and examined in terms of histopathology.
The introduced protocol created an irritant contact dermatitis model clinically and histopathologically.
In the present study, we introduced a new protocol using a mixture of dinitrochlorobenzene and acetone/olive oil to create an irritant contact dermatitis model. Mouse models have been extensively used to discover the complex mechanisms of irritant contact dermatitis and provide a preclinical platform before conducting clinical interventional research on humans to evaluate a new therapeutic approach. However, one should always look for models that cause the least pain and suffering in the animal and simultaneously are simple and reliable for the desired studies. Thus, our protocol is a new approach that can be effective and painless in creating a model of irritant contact dermatitis.
刺激性接触性皮炎是一种常见的炎症性皮肤病，其特征为皮肤屏障功能障碍、湿疹性皮炎和慢性瘙痒。这种疾病严重影响生活质量。鉴于目前的治疗方法并不理想，需要进行更广泛的研究以开发新的治疗方法。主要而言，需要一种小鼠模型来研究治疗该疾病的新药的有效性。
本研究旨在创建一种刺激性接触性皮炎的小鼠模型。
在本研究中，我们使用BALB/c雌性小鼠制备刺激性接触性皮炎的小鼠模型。为诱导刺激性接触性皮炎，我们使用二硝基氯苯与丙酮/橄榄油的混合物作为刺激物。应用10天后，分离小鼠皮肤组织并进行组织病理学检查。
所采用的方案在临床和组织病理学上创建了一个刺激性接触性皮炎模型。
在本研究中，我们引入了一种使用二硝基氯苯与丙酮/橄榄油混合物的新方案来创建刺激性接触性皮炎模型。小鼠模型已被广泛用于发现刺激性接触性皮炎的复杂机制，并在对人类进行临床干预研究以评估新的治疗方法之前提供一个临床前平台。然而，人们应该始终寻找在动物中引起最小疼痛和痛苦且同时对所需研究简单可靠的模型。因此，我们的方案是一种在创建刺激性接触性皮炎模型时有效且无痛的新方法。
