登革热病毒非结构蛋白 4A 中的一个 N 端两亲螺旋介导寡聚化，对复制至关重要。
An N-terminal amphipathic helix in dengue virus nonstructural protein 4A mediates oligomerization and is essential for replication.
机构信息
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
出版信息
J Virol. 2013 Apr;87(7):4080-5. doi: 10.1128/JVI.01900-12. Epub 2013 Jan 16.
Abstract
Dengue virus (DENV) causes dengue fever, a major health concern worldwide. We identified an amphipathic helix (AH) in the N-terminal region of the viral nonstructural protein 4A (NS4A). Disruption of its amphipathic nature using mutagenesis reduced homo-oligomerization and abolished viral replication. These data emphasize the significance of NS4A in the life cycle of the dengue virus and demarcate it as a target for the design of novel antiviral therapy.
摘要
登革热病毒（DENV）可引起登革热，这是全球范围内一个重大的健康关注点。我们在病毒非结构蛋白 4A（NS4A）的 N 端区域发现了一个两亲螺旋（AH）。通过突变破坏其两亲性质，可减少同源寡聚化并消除病毒复制。这些数据强调了 NS4A 在登革热病毒生命周期中的重要性，并将其确定为设计新型抗病毒治疗方法的靶标。
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