Pradhan V, Rajadhyaksha A, Mahant G, Surve P, Patwardhan M, Dighe S, Ghosh K
Department of Autoimmune Disorders, National Institute of Immunohematology, Indian Council of Medical Research, Mumbai, India.
Indian J Nephrol. 2012 Sep;22(5):353-7. doi: 10.4103/0971-4065.103911.
Systemic lupus erythematosus (SLE) is a prototype autoimmune disease, characterized by immune complex formation and systemic inflammation. Complement components such as C1q and mannose-binding lectin (MBL) play an important role in the clearance of immune complexes. Anti-C1q antibodies are associated with lupus nephritis and reduced levels of the complement components. The objective of this study was to detect anti-C1q antibodies in SLE patients and to evaluate their association with the complement components. Sixty SLE patients were included, of whom 75% had lupus nephritis (LN) and 25% were without renal manifestations (non-LN). The disease activity was assessed at the time of evaluation by the systemic lupus erythematosus disease activity index (SLEDAI). Anti-C1q antibodies, circulating immune complexes, and serum MBL levels were detected by enzyme-linked immunosorbent assay. The anti-C1q antibody prevalence was 58.3%. The LN patients showed 60% anti-C1q positivity with a higher percentage in membranoproliferative glomerulonephritis patients (51.9%). Anti-dsDNA positivity was slightly higher among the anti-C1q positives than in the anti-C1q negatives (65.7% vs. 60%). A higher percentage of reduced C3 and C4 levels was noted among the anti-C1q positives. The LN patients showed a higher percentage of low MBL levels among anti-C1q negatives than in the anti-C1q positives (61.1% vs. 55.6%). Non-LN patients showed a higher percentage of low MBL levels among anti-C1q positives than among anti-C1q negatives (87.5% vs. 57.1%). Anti-C1q antibodies were found in both LN and non-LN patients, but there was no correlation with the clinical severity of the disease.
系统性红斑狼疮（SLE）是一种典型的自身免疫性疾病，其特征为免疫复合物形成和全身性炎症。补体成分如C1q和甘露糖结合凝集素（MBL）在免疫复合物清除中起重要作用。抗C1q抗体与狼疮性肾炎及补体成分水平降低相关。本研究的目的是检测SLE患者中的抗C1q抗体，并评估其与补体成分的关联。纳入了60例SLE患者，其中75%有狼疮性肾炎（LN），25%无肾脏表现（非LN）。在评估时通过系统性红斑狼疮疾病活动指数（SLEDAI）评估疾病活动度。采用酶联免疫吸附测定法检测抗C1q抗体、循环免疫复合物和血清MBL水平。抗C1q抗体患病率为58.3%。LN患者抗C1q阳性率为60%，膜增生性肾小球肾炎患者中该比例更高（51.9%）。抗双链DNA阳性率在抗C1q阳性者中略高于抗C1q阴性者（65.7%对60%）。抗C1q阳性者中C3和C4水平降低的比例更高。LN患者中抗C1q阴性者低MBL水平的比例高于抗C1q阳性者（61.1%对55.6%）。非LN患者中抗C1q阳性者低MBL水平的比例高于抗C1q阴性者（87.5%对57.1%）。LN和非LN患者中均发现了抗C1q抗体，但与疾病的临床严重程度无相关性。
