Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
World J Gastroenterol. 2012 Dec 28;18(48):7251-61. doi: 10.3748/wjg.v18.i48.7251.
To investigate the relationship between c.343A>G and c.2216A>C polymorphism sites in the CDH17 gene and colorectal carcinoma.
Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study. Ninety-three peripheral venous blood samples, of approximately one milliliter from each patient, were collected between December 2009 and August 2010. The genomic DNA of these peripheral venous blood samples were extracted and purified using a Fermentas Genomic DNA Purification Kit (Fermentas, CA) according to the manufacturer's protocol. The single nucleotide polymorphisms (SNPs) of the liver-intestine cadherin (CDH17) gene c.343A>G and c.2216A>C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood samples from patients suffering with colorectal carcinoma. Typical samples that showed different migration bands in SSCP were confirmed by sequencing. Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method.
There was a significant association between the c.2216 A>C SNPs of the CDH17 gene and the tumor-node-metastasis (TNM) grade, as well as with lymph node status, in 93 peripheral venous blood samples from colorectal carcinoma patients. The genotype frequencies of A/C, A/A, and C/C were 12.90%, 33.33% and 53.76%, respectively. There was a significant correlation between lymph node metastasis, TNM grade, and the genotype distribution (P < 0.05). The C/C genotype raised the risk of lymph node metastasis and the TNM grade. There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes (P = 0.003 and P = 0.013, respectively). Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade. The difference between the TNM grades, as well as the lymph node metastasis of the two alleles, was statistically significant (P < 0.01).
The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma.
探讨钙黏蛋白 17（CDH17）基因 c.343A>G 和 c.2216A>C 多态性与结直肠癌的关系。
选取 2009 年 12 月至 2010 年 8 月郑州大学第一附属医院肿瘤科收治的 93 例非血缘关系的结直肠癌患者为研究对象，采集外周静脉血 1ml，采用 Fermentas Genomic DNA Purification Kit（Fermentas，CA）提取和纯化基因组 DNA。采用聚合酶链反应-单链构象多态性（PCR-SSCP）方法检测 93 例结直肠癌患者外周静脉血 CDH17 基因 c.343A>G 和 c.2216A>C 单核苷酸多态性（SNP）。PCR-SSCP 出现典型迁移带的样本通过测序进行确认，测序结果与 PCR-SSCP 法的基因型结果进行比较以验证正确性。
93 例结直肠癌患者外周静脉血 CDH17 基因 c.2216A>C SNP 与肿瘤-淋巴结-转移（TNM）分级和淋巴结状态有关。A/C、A/A 和 C/C 的基因型频率分别为 12.90%、33.33%和 53.76%。淋巴结转移、TNM 分级与基因型分布有显著相关性（P < 0.05）。C/C 基因型增加了淋巴结转移和 TNM 分级的风险。A/A 和 C/C 基因型的 TNM 分级和淋巴结转移差异有统计学意义（P = 0.003 和 P = 0.013）。携带 C 等位基因的结直肠癌患者有更高的淋巴结转移和更高的 TNM 分级风险。两个等位基因的 TNM 分级和淋巴结转移差异有统计学意义（P < 0.01）。
CDH17 基因 c.2216A>C SNP 可能与结直肠癌的预后有关。
