Kuraoka Kazuya, Oue Naohide, Yokozaki Hiroshi, Kitadai Yasuhiko, Ito Reiko, Nakayama Hirofumi, Yasui Wataru
Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8551, Japan.
Int J Oncol. 2003 Aug;23(2):421-7.
A C/A single nucleotide polymorphism (SNP) in the E-cadherin gene promoter has been shown to affect transcriptional efficiency in vitro. In this study, we analyzed the C/A SNP in 106 gastric carcinoma cases and 90 cancer-free controls compared with clinicopathological parameters. The genotype of C/C, C/A, and A/A was found in 61 (58%), 34 (32%) and 11 (10%) of 106 gastric carcinoma cases and in 32 (36%), 52 (58%) and 6 (6%) of 90 cancer-free controls, respectively. The genotype of C/C was significantly more frequent in gastric carcinoma cases than in cancer-free controls (p<0.05). The E-cadherin protein expression level in non-neoplastic gastric mucosa was higher in the C/C genotype than in the C/A. In gastric carcinoma cases, the genotype of C/C was significantly more frequent in the cases of poorly differentiated adenocarcinoma (31/44; 70%) than in well differentiated type (30/62; 48%). The C/C genotype was also significantly more frequent in the cases with deep invasion (51/74; 69%) and with lymph node metastasis (38/54; 70%) than in those confined superficially (10/32; 31%) and without lymph node metastasis (23/52; 44%), respectively (p<0.05). These results suggest that the C/A SNP in the E-cadherin promoter may be a good marker for malignancy of gastric carcinomas.
E-钙黏蛋白基因启动子中的一个C/A单核苷酸多态性（SNP）已被证明在体外会影响转录效率。在本研究中，我们分析了106例胃癌病例和90例无癌对照中的C/A SNP，并与临床病理参数进行了比较。在106例胃癌病例中，分别有61例（58%）、34例（32%）和11例（10%）的基因型为C/C、C/A和A/A；在90例无癌对照中，分别有32例（36%）、52例（58%）和6例（6%）的基因型为C/C、C/A和A/A。胃癌病例中C/C基因型的频率显著高于无癌对照（p<0.05）。非肿瘤性胃黏膜中C/C基因型的E-钙黏蛋白蛋白表达水平高于C/A基因型。在胃癌病例中，低分化腺癌（31/44；70%）中C/C基因型的频率显著高于高分化型（30/62；48%）。C/C基因型在深层浸润（51/74；69%）和有淋巴结转移（38/54；70%）的病例中的频率也分别显著高于浅表局限（10/32；31%）和无淋巴结转移（23/52；44%）的病例（p<0.05）。这些结果表明，E-钙黏蛋白启动子中的C/A SNP可能是胃癌恶性程度较好的标志物。
