腺苷脱氨酶与红细胞和血小板质膜的关联：一项运用光学显微镜和电子显微镜的免疫学研究。

Association of adenosine deaminase with erythrocyte and platelet plasma membrane: an immunological study using light and electron microscopy.

作者信息

Franco R, Aran J M, Colomer D, Matutes E, Vives-Corrons J L

机构信息

Department de Bioquímica i Fisiologia, Unitat de Bioquímica i Biologia Molecular A, Facultat de Química, Barcelona, Catalunya, Spain.

出版信息

J Histochem Cytochem. 1990 May;38(5):653-8. doi: 10.1177/38.5.2332624.

DOI:10.1177/38.5.2332624

PMID:2332624

Abstract

Adenosine deaminase has been localized in the plasma membrane of erythrocytes and platelets by means of immunological techniques using light and electron microscopy with cells in suspension. In erythrocytes, adenosine deaminase is associated with the external side of the plasma membrane. In platelets, the enzyme is associated with the external side of the plasma membrane, which is known to extend through the canalicular system of these cells. These results confirm our previous findings, based on biochemical studies, concerning the attachment of the enzyme to cell membranes.

摘要

通过使用光镜和电镜的免疫技术，在悬浮细胞中已将腺苷脱氨酶定位在红细胞和血小板的质膜上。在红细胞中，腺苷脱氨酶与质膜的外侧相关联。在血小板中，该酶与质膜的外侧相关联，已知质膜延伸穿过这些细胞的小管系统。这些结果证实了我们先前基于生化研究得出的关于该酶与细胞膜结合的发现。