Avian Viral Diseases Programme, The Pirbright Institute, Compton Laboratory, Newbury, Berkshire, United Kingdom.
PLoS One. 2013;8(1):e51799. doi: 10.1371/journal.pone.0051799. Epub 2013 Jan 9.
The generation of appropriate adaptive immune responses relies critically on dendritic cells, about which relatively little is known in chickens, a vital livestock species, in comparison with man and mouse. We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative PCR assays and monoclonal antibodies to study expression of DEC205 as well as CD83. The gene structure of DEC205 was identical to those of other species. Transcripts of both genes were found at higher levels in lymphoid tissues and the expression of DEC205 in normal birds had a characteristic distribution in the primary lymphoid organs. In spleen, DEC205 was seen on cells ideally located to trap antigen. In thymus it was found on cells thought to participate in the education of T cells, and in the bursa on cells that may be involved in presentation of antigen to B cells and regulation of B cell migration. The expression of DEC205 on cells other than antigen presenting cells (APC) is also described. Isolated splenocytes strongly expressing DEC205 but not the KUL01 antigen have morphology similar to mammalian dendritic cells and the distinct expression of DEC205 within the avian-specific Bursa of Fabricius alludes to a unique function in this organ of B cell diversification.
适当的适应性免疫反应的产生依赖于树突状细胞，与人类和小鼠相比，鸡作为一种重要的家畜物种，关于树突状细胞的了解相对较少。我们克隆并测序了鸡 DEC205 cDNA，并利用这些知识开发了定量 PCR 检测和单克隆抗体，以研究 DEC205 和 CD83 的表达。DEC205 的基因结构与其他物种相同。在淋巴组织中发现了这两种基因的转录本水平较高，正常鸟类 DEC205 的表达在初级淋巴器官中有特征性分布。在脾脏中，DEC205 存在于理想位置以捕获抗原的细胞上。在胸腺中，它存在于被认为参与 T 细胞教育的细胞上，在法氏囊中，它存在于可能参与向 B 细胞呈递抗原和调节 B 细胞迁移的细胞上。还描述了 DEC205 在抗原提呈细胞 (APC) 以外的细胞上的表达。强烈表达 DEC205 但不表达 KUL01 抗原的分离脾细胞具有类似于哺乳动物树突状细胞的形态，并且 DEC205 在禽类特异性法氏囊中独特表达暗示了其在 B 细胞多样化的这个器官中的独特功能。
