Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victorias Km. 0.6, Hermosillo, Sonora, Mexico.
Dev Comp Immunol. 2010 Jul;34(7):715-21. doi: 10.1016/j.dci.2010.02.010. Epub 2010 Mar 5.
Dendritic cells express a type cell-surface receptor, CD205 that plays a role in antigen capture and delivery to the endocytic pathway. Besides DCs, high CD205 expression is also detected on thymic epithelial cells but B cells, macrophages, and T cells have limited or no expression. CD205 has been characterized in several animal species except swine. The aim of this work was to characterize porcine CD205 and mRNA expression on different cells and tissues involved in immune responses. A complete porcine CD205 sequence of 5175bp was obtained from porcine thymus cDNA by PCR gene-walking strategy and this gene encoded a protein of 1723 amino acids. The multi-domain structure reported for murine, human, and bovine CD205 was also conserved in porcine with an overall amino acid identity of 74, 81, and 85%, respectively. Quantitative RT-PCR analysis of the CD205 mRNA profiles in normal porcine tissues and cells showed that thymus and Langerhans cells expressed the highest levels. Further characterization of porcine CD205 will lead to better understanding of the role of this receptor and development of contemporary strategies for antigen targeting to DCs in swine.
树突状细胞表达一种细胞表面受体 CD205，该受体在抗原捕获和递送至内吞途径中发挥作用。除树突状细胞外，高水平的 CD205 表达还可检测到在胸腺上皮细胞上，但 B 细胞、巨噬细胞和 T 细胞的表达有限或没有。除了猪以外，在几种动物物种中已经对 CD205 进行了描述。这项工作的目的是对参与免疫反应的不同细胞和组织中的猪 CD205 和 mRNA 表达进行特征分析。通过 PCR 基因行走策略从猪胸腺 cDNA 中获得了完整的 5175bp 猪 CD205 序列，该基因编码 1723 个氨基酸的蛋白质。报道的鼠、人、牛 CD205 的多结构域结构在猪中也得到了保守，分别具有 74%、81%和 85%的总体氨基酸同一性。对正常猪组织和细胞中 CD205 mRNA 谱的定量 RT-PCR 分析显示，胸腺和朗格汉斯细胞表达水平最高。对猪 CD205 的进一步特征分析将有助于更好地理解该受体的作用，并开发针对猪树突状细胞的当代抗原靶向策略。
