Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, Japan.
PLoS One. 2013;8(1):e54019. doi: 10.1371/journal.pone.0054019. Epub 2013 Jan 11.
In Arabidopsis thaliana the CPC-like MYB transcription factors [CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3 (ETC1, ETC2, ETC3/CPL3), TRICHOMELESS 1, 2/CPC-LIKE MYB 4 (TCL1, TCL2/CPL4)] and the bHLH transcription factors [GLABRA3 (GL3) and ENHANCER OF GLABRA 3 (EGL3)] are central regulators of trichome and root-hair development. We identified TRY and GL3 homologous genes from the tomato genome and named them SlTRY and SlGL3, respectively. Phylogenic analyses revealed a close relationship between the tomato and Arabidopsis genes. Real-time reverse transcription PCR analyses showed that SlTRY and SlGL3 were predominantly expressed in aerial parts of developing tomato. After transformation into Arabidopsis, CPC::SlTRY inhibited trichome formation and enhanced root-hair differentiation by strongly repressing GL2 expression. On the other hand, GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation. These results suggest that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation, and that a CPC-like R3 MYB may be a key common regulator of plant trichome and root-hair development.
在拟南芥中，CPC 样 MYB 转录因子（CAPRICE（CPC）、TRIPTYCHON（TRY）、ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3（ETC1、ETC2、ETC3/CPL3）、TRICHOMELESS 1, 2/CPC-LIKE MYB 4（TCL1、TCL2/CPL4））和 bHLH 转录因子（GLABRA3（GL3）和 ENHANCER OF GLABRA 3（EGL3））是毛状体和根毛发育的中央调控因子。我们从番茄基因组中鉴定出 TRY 和 GL3 同源基因，并分别命名为 SlTRY 和 SlGL3。系统发育分析表明，番茄和拟南芥基因之间存在密切关系。实时逆转录 PCR 分析显示，SlTRY 和 SlGL3 在番茄发育的地上部分中主要表达。转化到拟南芥后，CPC::SlTRY 通过强烈抑制 GL2 的表达来抑制毛状体的形成并增强根毛的分化。另一方面，GL3::SlGL3 转化对毛状体或非毛细胞的分化没有明显影响。这些结果表明，番茄和拟南芥部分利用相似的转录因子进行表皮细胞分化，并且 CPC 样 R3 MYB 可能是植物毛状体和根毛发育的关键共同调控因子。
