Nagao Kosuke, Tominaga Rumi
Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima, Japan.
Plant Signal Behav. 2020 Oct 2;15(10):1800198. doi: 10.1080/15592324.2020.1800198. Epub 2020 Aug 2.
CAPRICE (CPC), an R3-type MYB transcription factor, is known to promote root hair differentiation in the root epidermis of . CPC moves from non-hair cells to adjacent hair-forming cells. In contrast, we have previously shown that there is no movement of the CPC homologs, ENHANCER OF TRY AND CPC 1, 2, and 3 (ETC1, 2, and 3), and TRYPTICHON (TRY) between root epidermal cells. We also identified a tomato homolog of , named ().-introduced transgenic produced many root hairs, like -introduced transgenic . To clarify the cell-to-cell movement ability of the SlTRY protein, in this study, we observed the distribution of GFP fluorescence in transgenic . Unexpectedly, SlTRY moved from non-hair cells to adjacent root hair cells, like CPC, in root epidermis. SlTRY does not have the cell-to-cell movement sequence (S1) defined in CPC, and the mechanism of movement is still unknown. Further investigation is necessary to elucidate the mechanism of cell-to-cell movement of SlTRY. Our results will help in the further unraveling of the functions of these MYB transcription factors in determining cell fate.
CAPRICE（CPC）是一种R3型MYB转录因子，已知其可促进拟南芥根表皮中根毛的分化。CPC从非毛细胞移动到相邻的毛形成细胞。相比之下，我们之前已经表明，CPC同源物TRY和CPC的增强子1、2和3（ETC1、2和3）以及TRYPTICHON（TRY）在根表皮细胞之间不会移动。我们还鉴定出了番茄中的一个CPC同源物，命名为SlTRY。导入SlTRY的转基因拟南芥产生了许多根毛，就像导入CPC的转基因拟南芥一样。为了阐明SlTRY蛋白的细胞间移动能力，在本研究中，我们观察了GFP荧光在导入SlTRY的转基因拟南芥中的分布。出乎意料的是，在拟南芥根表皮中，SlTRY像CPC一样从非毛细胞移动到相邻的根毛细胞。SlTRY不具有CPC中定义的细胞间移动序列（S1），其移动机制仍然未知。需要进一步研究以阐明SlTRY细胞间移动的机制。我们的结果将有助于进一步揭示这些MYB转录因子在决定细胞命运中的功能。
