Ghalayani Parichehr, Jahanshahi Gholamreza, Saberi Zahra
Department of Oral and Maxillofacial Medicine and Torabinejad Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2012;1:52. doi: 10.4103/2277-9175.100161. Epub 2012 Aug 28.
The objective of this study was to assess mast cells and TNF-α in oral lichen planus (OLP) and oral lichenoid reactions (OLR) patients as diagnostic marker to the differential diagnosis of OLP and OLR diseases.
In this cross-sectional study, samples were obtained from 30 OLP and 30 OLR patients, between June 2010 and March 2011 in Dental clinic of the University of Isfahan, Iran. Mast cells in the reticular layer of the lamina propria for samples were evaluated using toluidine blue method and immunohistochemical technique. The clinical relevant data taken into account were: demographical data, total number and degranulated mast cells, ratio of degranulated mast cells and TNF-α positive degranulated mast cells.
In OLP and OLR, the total mast cells were 21.2 ± 7.9 and 20.3 ± 6.8, degranulated mast cells were 15.5 ± 6.9 and 19.2 ± 6.9, ratio of degranulated mast cells to total mast cells were 0.716 ± 0.067 and 0.946 ± 0.081, and TNF-α positive degranulated mast cells were 13.6 ± 6.3 and 17.1 ± 6.04, respectively. There was no significant difference for the total mast cells. But degranulated mast cells, ratio of degranulated mast cells and TNF-α positive degranulated mast cells in OLR were significantly higher than OLP patients.
Our results showed that the degranulated mast cells, ratio of degranulated mast cells and TNF-α in OLR was significantly more than OLP patients and these may be able to be used as diagnostic markers to the differential diagnosis of OLP and OLR.
本研究的目的是评估口腔扁平苔藓（OLP）和口腔苔藓样反应（OLR）患者中的肥大细胞和肿瘤坏死因子-α（TNF-α），作为OLP和OLR疾病鉴别诊断的诊断标志物。
在这项横断面研究中，于2010年6月至2011年3月期间在伊朗伊斯法罕大学牙科诊所从30例OLP患者和30例OLR患者中获取样本。使用甲苯胺蓝法和免疫组织化学技术评估样本固有层网状层中的肥大细胞。考虑的临床相关数据包括：人口统计学数据、肥大细胞总数和脱颗粒肥大细胞、脱颗粒肥大细胞比例以及TNF-α阳性脱颗粒肥大细胞。
在OLP和OLR中，肥大细胞总数分别为21.2±7.9和20.3±6.8，脱颗粒肥大细胞分别为15.5±6.9和19.2±6.9，脱颗粒肥大细胞与肥大细胞总数的比例分别为0.716±0.067和0.946±0.081，TNF-α阳性脱颗粒肥大细胞分别为13.6±6.3和17.1±6.04。肥大细胞总数无显著差异。但OLR中的脱颗粒肥大细胞、脱颗粒肥大细胞比例和TNF-α阳性脱颗粒肥大细胞均显著高于OLP患者。
我们的结果表明，OLR中的脱颗粒肥大细胞、脱颗粒肥大细胞比例和TNF-α显著多于OLP患者，这些可能可用作OLP和OLR鉴别诊断的诊断标志物。
