Wu Jiang, Huang De-ying, Cheng Jie, Chen Xue-ling, Xiang Qiang
Wuhan Municipal Healthcare Center for Women and Children, Wuhan, China.
Zhonghua Yi Xue Za Zhi. 2012 Nov 6;92(41):2938-42.
To explore the response to nociceptive stimuli in spinal cord of neonatal rat and observe the electrical stimulation of sciatic nerve on synaptic plasticity of spinal dorsal horn, hippocampal CA1 region and spinal c-fos, hippocampal CA1 region expression in neonatal rats.
The rats were divided into neonatal and adult groups. The evoked potentials of left spinal dorsal horn from T13-L1 and right hippocampal CA1 region were recorded. After conditional electric stimulations, the potential amplitudes were recorded. When the incubation period reached 120 min, D-AP5 was added to spinal cord of rats and resulting changes in field potential and c-fos expression were recorded.
Long-term potentiation (LTP) in neonatal rats was mainly evoked by A-type nerve fibers whereas LTP in adult rats was mainly evoked by C-type nerve fibers. C-fos expression was significantly increased in superficial, deep layers of spinal dorsal horn, ventral horn and hippocampal CA1 region in neonatal rats.
Pain signals change with age.
探讨新生大鼠脊髓对伤害性刺激的反应，观察坐骨神经电刺激对新生大鼠脊髓背角、海马CA1区突触可塑性及脊髓c-fos、海马CA1区表达的影响。
将大鼠分为新生组和成年组。记录T13-L1左侧脊髓背角和右侧海马CA1区的诱发电位。给予条件电刺激后，记录电位幅度。潜伏期达到120分钟时，向大鼠脊髓中加入D-AP5，记录场电位和c-fos表达的变化。
新生大鼠的长时程增强（LTP）主要由A型神经纤维诱发，而成年大鼠的LTP主要由C型神经纤维诱发。新生大鼠脊髓背角浅层、深层、腹角及海马CA1区c-fos表达明显增加。
疼痛信号随年龄变化。
