Pediatrics Center of Excellence, Children's Medical Center.
Eur Cytokine Netw. 2012 Oct-Dec;23(4):163-5. doi: 10.1684/ecn.2012.0323.
Tumor necrosis factor-alpha (TNF-α) could be considered as potential biomarkers in atopic dermatitis (AD), while its level could be influenced by cytokine single gene polymorphisms (SNP). This study was performed in 89 pediatric patients with AD and 137 controls to assess polymorphisms of the TNF-α gene at positions -308 and -238, using the polymerase chain reaction and the sequence-specific primers method. The highest positive allelic association that made the patients susceptible to AD was seen for TNF-α -238/G (p<0.001) and TNF-α -308/G (p = 0.003). The GG genotypes at TNF-α -238 and TNF-α -308, were both significantly higher in the patients with AD, compared to the controls (p<0.01). The GG haplotype at TNF-α (-308,-238) was seen in 92.7% of the patients, which was significantly higher than the controls (p<0.001), while a negative haplotypic association with AD was seen for TNF-α (-308, -238) AG and GA (p<0.01). This study showed that the AG genotype of TNF-α -308, associated with a high production of cytokines, was significantly decreased in patients with AD, while the low-producing GG genotype, which could lead to low production of TNF-α, was over-expressed in the atopic patients.
肿瘤坏死因子-α（TNF-α）可被视为特应性皮炎（AD）的潜在生物标志物，而其水平可能受到细胞因子单核苷酸多态性（SNP）的影响。本研究在 89 名儿科 AD 患者和 137 名对照中进行，使用聚合酶链反应和序列特异性引物方法评估 TNF-α 基因在位置-308 和-238 的多态性。TNF-α-238/G（p<0.001）和 TNF-α-308/G（p=0.003）的最高阳性等位基因关联使患者易患 AD。TNF-α-238 和 TNF-α-308 的 GG 基因型在 AD 患者中均显著高于对照组（p<0.01）。TNF-α（-308，-238）的 GG 单倍型在 92.7%的患者中可见，明显高于对照组（p<0.001），而 TNF-α（-308，-238）AG 和 GA 与 AD 呈负相关（p<0.01）。本研究表明，TNF-α-308 的 AG 基因型与细胞因子的高产量相关，在 AD 患者中显著降低，而低产量的 GG 基因型可能导致 TNF-α 的低产量，在特应性患者中过度表达。
