[大鼠丘脑前核电刺激时海马细胞外液谷氨酸和γ-氨基丁酸的变化]
[Changes of glutamate and γ-aminobutyric acid of extracellular fluid in hippocampus during electrical stimulation of anterior nucleus thalamus in rats].
作者信息
Chen Ning, Meng Fan-gang, Zhang Jian-guo, Yang An-chao, Liu Huan-guang, Hu Wen-han, Meng Da-wei, Zhang Xin, Liu Chong, Ge Yan
机构信息
Beijing Tiantan Hospital, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2012 Dec 18;92(47):3371-3.
OBJECTIVE
To explore the changes of extracellular glutamate (Glu) and γ-aminobutyric acid (GABA) in hippocampus during the high-frequency stimulation of anterior nucleus thalamus (ANT) in epileptic rats.
METHODS
A total of 30 rats were divided equally into 2 groups:epilepsy model and normal control (n = 15 each). Concentric bipolar electrodes were stereotaxically implanted in bilateral ANT. A high-frequency stimulation of 130 Hz was applied in the epilepsy group. Microdialysis probes were unilaterally lowered into hippocampus. The concentrations of Glu and GABA in dialysate samples were determined by high-performance liquid chromatography (HPLC).
RESULTS
Electrical stimulation of ANT induced an increase of GABA and a decrease of Glu in hippocampus.
CONCLUSION
An increase of GABA and a decrease of Glu during the electrical stimulation of ANT play an important role in the therapy of epilepsy.
目的
探讨癫痫大鼠丘脑前核（ANT）高频刺激期间海马细胞外谷氨酸（Glu）和γ-氨基丁酸（GABA）的变化。
方法
将30只大鼠平均分为2组：癫痫模型组和正常对照组（每组n = 15）。采用立体定位法将同心双极电极植入双侧ANT。癫痫组给予130 Hz的高频刺激。将微透析探针单侧插入海马。采用高效液相色谱法（HPLC）测定透析液样本中Glu和GABA的浓度。
结果
ANT电刺激诱导海马中GABA增加和Glu减少。
结论
ANT电刺激期间GABA增加和Glu减少在癫痫治疗中起重要作用。
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