Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
Dig Dis Sci. 2013 Apr;58(4):1004-9. doi: 10.1007/s10620-012-2516-6. Epub 2013 Jan 18.
Nonalcoholic fatty liver disease (NAFLD) is strongly associated with obesity, hyperlipidemia, and type 2 diabetes mellitus. Several studies have found that fat mass and the obesity-associated (FTO) gene is linked to obesity. The aim of this work is to investigate the expression and function of FTO in liver with lipid metabolism diseases.
We investigated the basal FTO expression in an NAFLD rat model and compared it with control subjects. The function of FTO in lipid metabolism was further studied in L02 cells through overexpression experiments.
A significant increase in FTO mRNA and protein levels was found in the NAFLD group. In addition, the FTO levels were positively associated with malondialdehyde and superoxide dismutase concentrations. FTO overexpression in L02 cells enhanced lipogenesis and oxidative stress.
This study demonstrates that increased FTO levels in the liver are involved in oxidative stress and lipid deposition, which characterize NAFLD.
非酒精性脂肪性肝病（NAFLD）与肥胖、高血脂和 2 型糖尿病密切相关。多项研究发现，脂肪量和肥胖相关（FTO）基因与肥胖有关。本研究旨在探讨 FTO 在伴有脂代谢疾病的肝脏中的表达和功能。
我们检测了 NAFLD 大鼠模型中的基础 FTO 表达，并与对照组进行比较。通过过表达实验进一步研究了 FTO 在 L02 细胞中对脂代谢的作用。
NAFLD 组 FTO mRNA 和蛋白水平显著升高。此外，FTO 水平与丙二醛和超氧化物歧化酶浓度呈正相关。L02 细胞中 FTO 的过表达增强了脂肪生成和氧化应激。
本研究表明，肝脏中 FTO 水平的升高与 NAFLD 的特征性氧化应激和脂质沉积有关。
