Logistics University of Chinese People's Armed Police Force, Tianjin 300162, China.
Sci China Life Sci. 2013 Feb;56(2):181-8. doi: 10.1007/s11427-012-4404-x. Epub 2013 Jan 18.
The skin is the largest organ of the body and is a potential route of exposure to sunscreens and cosmetics containing nanoparticles; however, the permeability of the skin to these nanoparticles is currently unknown. In this paper, we studied the transdermal delivery capacity through mouse skin of water-soluble CdSeS quantum dots (QDs) and the deposition of these QDs in the body. QD solution was coated onto the dorsal hairless skin of male ICR mice. Fluorescence microscopy and transmission electron microscopy (TEM) were used to observe the distribution of QDs in the skin and organs, and inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure the (111)Cd content to indicate the concentration of QDs in plasma and organs. Experimental results indicate that QDs can penetrate into the dermal layer and are limited to the uppermost stratum corneum layers and the hair follicles. Through blood circulation, QDs deposit mostly in liver and kidney and are difficult to clear. (111)Cd concentration was greater than 14 ng g(-1) in kidney after 120 h after 0.32 nmol QDs was applied to a mouse. These results suggest that QDs have in vivo transdermal delivery capacity through mouse skin and are harmful to the liver and kidney.
皮肤是人体最大的器官，也是暴露于含有纳米粒子的防晒霜和化妆品的潜在途径；然而，皮肤对这些纳米粒子的通透性目前尚不清楚。在本文中，我们研究了水相 CdSeS 量子点（QD）通过小鼠皮肤的经皮传递能力以及这些 QD 在体内的沉积。QD 溶液涂覆在雄性 ICR 小鼠的背部无毛皮肤上。荧光显微镜和透射电子显微镜（TEM）用于观察 QD 在皮肤和器官中的分布，电感耦合等离子体质谱（ICP-MS）用于测量（111）Cd 含量以指示 QD 在血浆和器官中的浓度。实验结果表明，QD 可以穿透真皮层，仅限于最上层的角质层和毛囊。通过血液循环，QD 主要沉积在肝脏和肾脏中，并且难以清除。在向小鼠施用 0.32 nmol QD 后 120 h，肾脏中的（111）Cd 浓度大于 14 ng g(-1)。这些结果表明 QD 具有通过小鼠皮肤的体内经皮传递能力，对肝脏和肾脏有害。
