Fiolo Kateli, Zanardi Cibele Esteves, Salvadego Marizete, Bertuzzo Carmem Silvia, Amaral Eliana, Calil Roseli, Levy Carlos Emilio
Programa de Saúde da Criança e do Adolescente, Universidade Estadual de Campinas - UNICAMP, Campinas, SP, Brasil.
Rev Bras Ginecol Obstet. 2012 Dec;34(12):544-9. doi: 10.1590/s0100-72032012001200003.
To describe the epidemiological cases and microbiological profile of Streptococcus agalactiae serotypes isolated from infected newborns of a Women's Health Reference Centre of Campinas, São Paulo, Brazil.
Cross-sectional laboratory survey conducted from January 2007 to December 2011. The newborns' strains, isolated from blood and cerebrospinal fluid samples, were screened by hemolysis on blood ágar plates, Gram stain, catalase test, CAMP test, hippurate hydrolysis or by microbiological automation: Vitek 2 BioMerieux®. They were typed by PCR, successively using specific primers for species and nine serotypes of S. agalactiae.
Seven blood samples, one cerebrospinal fluid sample and an ocular sample, were isolated from nine newborns with infections caused by S. agalactiae, including seven cases of early onset and two of late onset. Only one of these cases was positive for paired mother-child samples. Considering that 13,749 deliveries were performed during the study period, the incidence was 0.5 cases of GBS infections of early onset per 1 thousand live births (or 0.6 per 1 thousand, including two cases of late onset) with 1, 3, 2, zero and 3 cases (one early and two late onset cases), respectively, for the years from 2007 to 2011. It was possible to apply PCR to seven of nine samples, two each of serotypes Ia and V and three of serotype III, one from a newborn and the other two from a paired mother-child sample.
Although the sample was limited, the serotypes found are the most prevalent in the literature, but different from the other few Brazilian studies available, except for type Ia.
描述从巴西圣保罗坎皮纳斯妇女健康参考中心感染新生儿中分离出的无乳链球菌血清型的流行病学病例和微生物学特征。
2007年1月至2011年12月进行横断面实验室调查。从血液和脑脊液样本中分离出的新生儿菌株，通过血琼脂平板上的溶血试验、革兰氏染色、过氧化氢酶试验、CAMP试验、马尿酸盐水解试验或微生物自动化检测：Vitek 2 BioMerieux®进行筛选。通过PCR进行分型，依次使用针对无乳链球菌物种和九种血清型的特异性引物进行检测。
从9例由无乳链球菌引起感染的新生儿中分离出7份血液样本、1份脑脊液样本和1份眼部样本，其中包括7例早发型和2例晚发型。这些病例中只有1例母婴配对样本呈阳性。考虑到研究期间共进行了13749例分娩，早发型GBS感染的发病率为每1000例活产中有0.5例（或每1000例中有0.6例，包括2例晚发型），2007年至2011年分别为1例、3例、2例、0例和3例（1例早发型和2例晚发型）。9份样本中有7份能够进行PCR检测，血清型Ia和V各2份，血清型III 3份，1份来自新生儿，另外2份来自母婴配对样本。
尽管样本有限，但所发现的血清型是文献中最常见的，但与巴西其他少数现有研究不同，除了Ia型。
