Wolny Katarzyna, Gołda-Matuszak Ewa
Katedra Mikrobiologii, Uniwersytet Rolniczy im. Hugona Kołłataja w Krakowie.
Med Dosw Mikrobiol. 2010;62(2):141-51.
The main aim of my research: to determine the frequency of colonisation Streptococcus agalactiae from productive women's vagina, an evaluation of usefulness microbiological diagnostic methods to detect GBS, to define serotype of analysed strains of S. agalactiae. After all, I tried to define fenotypic differential, biochemical and antimicrobial susceptibility between GBS with and without hemolysis. All of strains S. agalactiae (n = 380) belong to bacteria Gram(+), they had B serologic group and didn't produce catalase. On the basis of TSA+5% sheep blood streptococcus with beta-hemolysis grew like a small, grey and shiny colonies with a narrow, bright ring. On the same base we had S. agalactiae without beta-hemolysis, in examine material--6% (n = 22). On the basis of Strepto B ID S. agalactiae grew like a small, round red colonies and on the base Granada agar like an orange, white colonies. The level of colonisation S. agalactiae was 22% (380GBS/1727women). Identification of analysed strains of S. agalactiae was made by test API 20 Strep. The susceptibility was examined to ampicilin, azithromycin, erythromycin, clindamycin, chloramphenicol, doxycyclin, cotrimoxasol, ciprofloxacin. Serotypes III (50%), Ia (18%) and V (14%) prevailed.
确定有生育能力女性阴道中无乳链球菌的定植频率，评估微生物学诊断方法检测B群链球菌（GBS）的效用，确定所分析的无乳链球菌菌株的血清型。毕竟，我试图确定溶血和不溶血GBS之间的表型差异、生化特性及抗菌药敏情况。所有无乳链球菌菌株（n = 380）均属于革兰氏阳性菌，具有B血清群且不产生过氧化氢酶。在TSA + 5%羊血培养基上，β溶血的链球菌生长为小的、灰色且有光泽的菌落，周围有狭窄、明亮的晕环。在相同培养基上，不产生β溶血的无乳链球菌在检测材料中占6%（n = 22）。基于B群链球菌鉴定试验，无乳链球菌生长为小的圆形红色菌落，在格拉纳达琼脂培养基上生长为橙色、白色菌落。无乳链球菌的定植水平为22%（380株GBS / 1727名女性）。通过API 20 Strep试验对所分析的无乳链球菌菌株进行鉴定。检测了其对氨苄西林、阿奇霉素、红霉素、克林霉素、氯霉素、多西环素、复方新诺明、环丙沙星的药敏情况。血清型III（50%）、Ia（18%）和V（14%）占主导。
