School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China.
Biomed Microdevices. 2013 Jun;15(3):445-51. doi: 10.1007/s10544-013-9744-1.
The design, fabrication and test of a microfluidic cell trapping device to measure single cell exocytosis were reported. Procedures on the patterning of double layer template based on repetitive standard photolithography of AZ photoresist were investigated. The replicated poly(dimethyl siloxane) devices with 2.5 μm deep channels were proved to be efficient for stopping cells. Quantal exocytosis measurement can be achieved by targeting single or small clumps of chromaffin cells on top of the 10 μm × 10 μm indium tin oxide microelectrodes arrays with the developed microdevice. And about 72 % of the trapping sites can be occupied by cells with hydrodynamic trapping method and the recorded amperometric signals are comparable to the results with traditional carbon fiber microelectrodes. The method of manufacturing the microdevices is simple, low-cost and easy to perform. The manufactured device offers a platform for the high throughput detection of quantal catecholamine exocytosis from chromaffin cells with sufficient sensitivity and broad application.
报道了一种用于测量单细胞胞吐作用的微流控细胞捕获装置的设计、制造和测试。研究了基于重复标准 AZ 光刻胶光刻的双层模板的图案化过程。具有 2.5μm 深通道的复制聚二甲基硅氧烷器件被证明可以有效地阻止细胞。通过使用开发的微器件，可将单个或小簇嗜铬细胞靶向到 10μm×10μm 的铟锡氧化物微电极阵列的顶部，实现量子胞吐作用的测量。约 72%的捕获位点可被细胞占据，采用流体力学捕获方法记录的安培信号与传统碳纤维微电极的结果相当。微器件的制造方法简单、低成本且易于实施。所制造的器件为高通量检测嗜铬细胞中儿茶酚胺的量子胞吐作用提供了一个平台，具有足够的灵敏度和广泛的应用。
