使用微芯片进行量子胞吐的电化学测量。
Electrochemical measurement of quantal exocytosis using microchips.
机构信息
Department of Bioengineering, University of Missouri, Columbia, MO, USA.
Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO, USA.
出版信息
Pflugers Arch. 2018 Jan;470(1):97-112. doi: 10.1007/s00424-017-2063-2. Epub 2017 Sep 2.
Abstract
Carbon-fiber electrodes (CFEs) are the gold standard for quantifying the release of oxidizable neurotransmitters from single vesicles and single cells. Over the last 15 years, microfabricated devices have emerged as alternatives to CFEs that offer the possibility of higher throughput, subcellular spatial resolution of exocytosis, and integration with other techniques for probing exocytosis including microfluidic cell handling and solution exchange, optical imaging and stimulation, and electrophysiological recording and stimulation. Here we review progress in developing electrochemical electrode devices capable of resolving quantal exocytosis that are fabricated using photolithography.
摘要
碳纤维电极 (CFEs) 是量化单囊泡和单个细胞中可氧化神经递质释放的金标准。在过去的 15 年中，微加工设备作为 CFEs 的替代品出现，它们具有更高的通量、亚细胞分泌的空间分辨率以及与其他探测分泌技术集成的可能性，包括微流控细胞处理和溶液交换、光学成像和刺激以及电生理记录和刺激。在这里，我们回顾了使用光刻技术制造的能够解析量子分泌的电化学电极设备的发展进展。
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