Shao Chenren, Devoe Don L
Department of Mechanical Engineering, University of Maryland, College Park, MD, USA.
Methods Mol Biol. 2013;949:55-63. doi: 10.1007/978-1-62703-134-9_4.
Electroosmotic flow (EOF) is an electrokinetic flow control technique widely used in microfluidic systems for applications including direct electrokinetic pumping, hydrodynamic pressure generation, and counterflow for microfluidic separations. During EOF, an electric field is applied along the length of a microchannel containing an electrolyte, with mobile ions near the charged microchannel walls experiencing a Coulomb force due to electrostatic interactions with the applied electric field that leads to bulk solution movement. The goal of this laboratory is to experimentally determine the fixed channel surface charge (zeta potential) and electroosmotic mobility associated with a given microchannel substrate material and buffer solution, using a simple current monitoring method to measure the average flow velocity within the microchannel. It is a straightforward experiment designed to help students understand EOF physics while gaining hands-on experience with basic world-to-chip interfacing. It is well suited to a 90-min laboratory session for up to 12 students with minimal infrastructure requirements.
电渗流（EOF）是一种电动流控制技术，广泛应用于微流体系统，用于直接电动泵送、流体动力压力产生以及微流体分离中的逆流等应用。在电渗流过程中，沿着包含电解质的微通道长度施加电场，带电微通道壁附近的移动离子由于与外加电场的静电相互作用而受到库仑力，从而导致本体溶液移动。本实验室的目标是使用一种简单的电流监测方法来测量微通道内的平均流速，通过实验确定与给定微通道基底材料和缓冲溶液相关的固定通道表面电荷（zeta电位）和电渗迁移率。这是一个简单直接的实验，旨在帮助学生理解电渗流物理原理，同时获得基本的芯片与外界连接的实践经验。它非常适合90分钟的实验室课程，最多可供12名学生使用，基础设施要求极低。
