Kowalewski Daniel J, Stevanović Stefan
Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.
Department of Immunology, University of Tübingen, Interfaculty Institute for Cell Biology, Tübingen, Germany.
Methods Mol Biol. 2013;960:145-157. doi: 10.1007/978-1-62703-218-6_12.
The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this chapter we briefly review the existing strategies for the analysis of MHC ligandomes and provide an in-depth protocol for the immunoaffinity purification of MHC class I presented peptides from primary tissues or cells.
大规模鉴定主要组织相容性复合体（MHC）I类呈递的肽段，对于深入了解免疫识别的基本规则必不可少，同时也是确定疾病免疫治疗潜在靶点的宝贵工具。在本章中，我们简要回顾了分析MHC配体组的现有策略，并提供了从原代组织或细胞中免疫亲和纯化MHC I类呈递肽段的详细方案。
