Hashmi Satwat, Al-Salam Suhail
Department of Pathology, Faculty of Medicine & Health Sciences, United Arab Emirates University ALAIN PO Box 17666, UAE.
Int J Clin Exp Pathol. 2013;6(2):249-57. Epub 2013 Jan 15.
Myocardial infarction (MI) is the most frequent diagnosis made in majority of sudden death cases subjected to clinical and medicolegal autopsies. When sudden death occurs at a very early stage of MI, traditional macroscopic examination, or histological stains cannot easily detect the myocardial changes. For this reason we propose a new method for detecting MI at an early stage. Murine model of MI was used to induce MI through permanent ligation of left anterior descending branch of left coronary artery. Five groups of C57B6/J mice were used for inducing MI, which includes 20 minutes, 30 minutes, one hour, four hours and 24 hours post MI groups. One naïve group and sham-operated groups were used as controls. There is loss of dystrophin membranous staining in cardiac myocytes occurs as early as 20 minutes post myocardial infarction. This can be used as a novel method to diagnose early myocardial infarction in post mortem cases where diagnosis is unclear. In conclusion, evaluation of immunohistochemical expression of dystrophin represents a highly sensitive method for detecting early myocardial infarction due to the loss of staining in the infarcted areas. Dystrophin immunostaining can also be used to assess myocardial architecture.
心肌梗死（MI）是大多数接受临床和法医学尸检的猝死病例中最常见的诊断结果。当猝死发生在心肌梗死的极早期时，传统的宏观检查或组织学染色不易检测到心肌变化。因此，我们提出一种早期检测心肌梗死的新方法。采用心肌梗死小鼠模型，通过永久结扎左冠状动脉前降支诱导心肌梗死。使用五组C57B6/J小鼠诱导心肌梗死，包括心肌梗死后20分钟、30分钟、1小时、4小时和24小时组。一组未处理组和假手术组作为对照。心肌梗死后20分钟，心肌细胞中肌营养不良蛋白膜染色就开始丢失。这可作为一种新方法，用于诊断死后诊断不明确的早期心肌梗死病例。总之，由于梗死区域染色缺失，评估肌营养不良蛋白的免疫组化表达是检测早期心肌梗死的一种高度敏感的方法。肌营养不良蛋白免疫染色还可用于评估心肌结构。
