Hou Haijuan, Zhang Fan, Zhao Meilin, Cao Guifen, Huang Haiyun, Huang Ping
Dept. of Periodontology, The Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing Research Center for Oral Diseases and Biomedical Science, Chongqing 401147, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2012 Dec;30(6):628-31.
To study the relationship between endothelial nitric oxide synthase (eNOS) Glu298Asp gene polymorphism and chronic periodontitis (CP) with type 2 diabetes mellitus (T2DM).
DNA from patients' buccal swabs of CP, CP with T2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively.
The distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences (chi2 = 18.503, P = 0.005), and the gene frequency showed statistical differences (chi2 = 8.243, P = 0.041). Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% confidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant.
Based on these findings, there are some association between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.
研究内皮型一氧化氮合酶（eNOS）Glu298Asp基因多态性与2型糖尿病（T2DM）伴慢性牙周炎（CP）之间的关系。
分别从CP患者、T2DM伴CP患者、T2DM患者及健康者的颊黏膜拭子中分离提取DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估eNOS Glu298Asp基因多态性。
CP组、T2DM组、T2DM伴CP组和健康组中eNOS Glu298Asp基因型分布存在统计学差异（χ2 = 18.503，P = 0.005），基因频率也存在统计学差异（χ2 = 8.243，P = 0.041）。健康组与T2DM伴CP组相比，基因型T的OR值为0.962，95%置信区间为0.737至1.256，表明T可能是一个保护因素。而基因型G的OR值为1.043，95%置信区间为0.781至1.391，表明G可能是一个危险因素。然而，T和G均无统计学意义。
基于这些发现，eNOS Glu298Asp多态性与CP组、T2DM组、T2DM伴CP组的发病风险之间存在一定关联。
