Mirfeizi Mani, Hasanzad Mandana, Sattari Mahshid, Afshari Mahdi, Abbasi Davood, Ajoodani Zarin, Sheykheslam Ali Bani
Department of Midwifery, College of Nursing & Midwifery, Islamic Azad University, Karaj Branch, Moazen Blvd and Esteglal Blvd Rajaeishahr, Karaj, Alborz, 1916893813 Iran.
2Medical Genomics Research Center, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran.
J Diabetes Metab Disord. 2018 Aug 6;17(2):123-127. doi: 10.1007/s40200-018-0348-4. eCollection 2018 Dec.
Gestational diabetes mellitus (GDM) is the most popular metabolic disease during pregnancy. The aim of the present study was to investigate any possible association between Glu298Asp and I/D gene polymorphisms and the risk of GDM in a group of Iranian pregnant women.
In this case-control study 204 pregnant women were recruited (94 cases and 110 controls). Genomic DNA was isolated from whole blood and genotyping was performed by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR- RFLP) and only PCR for and polymorphisms respectively.
Frequencies of GT and TT genotype of polymorphism among women with and without GDM were 67.90% vs. 74.47 and 7.41% vs. 8.51% respectively ( = 0.4). Corresponding figures for DD genotype of polymorphism among GDM patients was more than that in healthy women (51.65% vs. 63.81% respectively). Conversely, ACE heterozygote genotype was more common in diabetic women (35.16% vs. 26.67% respectively). Although these differences were not statistically significant ( = 0.2).
Our study showed that there is no association between the presence of and gene polymorphisms and developing gestational diabetes mellitus among pregnant women in our population. Further longitudinal and multicenter studies should be carried out to assess the exact metabolic effects of these polymorphisms.
妊娠期糖尿病（GDM）是孕期最常见的代谢性疾病。本研究旨在调查一组伊朗孕妇中Glu298Asp和I/D基因多态性与GDM风险之间的任何可能关联。
在这项病例对照研究中，招募了204名孕妇（94例病例和110例对照）。从全血中分离基因组DNA，分别通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和仅PCR对和多态性进行基因分型。
GDM患者和非GDM患者中多态性的GT和TT基因型频率分别为67.90%对74.47%和7.41%对8.51%（=0.4）。GDM患者中多态性的DD基因型相应数字高于健康女性（分别为51.65%对63.81%）。相反，ACE杂合子基因型在糖尿病女性中更常见（分别为35.16%对26.67%）。尽管这些差异无统计学意义（=0.2）。
我们的研究表明，在我们的人群中，孕妇中基因多态性的存在与妊娠期糖尿病的发生之间没有关联。应进行进一步的纵向和多中心研究，以评估这些多态性的确切代谢影响。
