USDA-ARS Biological Integrated Pest Management Research, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853-2901, USA.
Fungal Biol. 2013 Jan;117(1):1-12. doi: 10.1016/j.funbio.2012.10.001. Epub 2012 Nov 22.
The entomopathogenic anamorphic genus Evlachovaea was described to differ from other fungi in forming its conidia obliquely to the axis of the conidiogenous cell and with successive conidia having alternate orientations with a zipper- or chevron-like arrangement resulting in flat, ribbon-like chains. Morphological and molecular studies of six Evlachovaea-like isolates baited from Central Brazilian soils using Triatoma infestans (a vector of Chagas disease) and of other entomopathogens with Evlachovaea-like conidiogenesis led to a re-evaluation of the status of this little known fungal genus. The Brazilian isolates formed two distinct groups based on gene sequences for both the internal transcribed spacer (ITS) and translation elongation factor (EF-1α) genes, morphology, and growth patterns; both groups also differed from the type species, Evlachovaea kintrischica. More detailed studies of these fungi indicated that the alternatingly oblique orientations of forming conidia are neither a stable nor invariant character (even on single phialides). Furthermore, the molecular cladistic analysis unambiguously placed the Evlachovaea isolates firmly within the genus Isaria (Hypocreales: Cordycipitaceae). The ITS sequences of E. kintrischica were very similar or even identical to those of Isaria amoenerosea and Isaria cateniobliqua, thereby suggesting that E. kintrischica is a synonym of one of these species, and that the genus Evlachovaea must be treated as a later synonym of Isaria, which must now be recognized to include several highly divergent modes of conidiogenesis. These taxonomic findings are discussed in the context of dramatic changes recently imposed on the nomenclatural standards used to determine the correct names of all pleomorphic fungi.
Entomopathogenic anamorphic genus Evlachovaea was described to differ from other fungi in forming its conidia obliquely to the axis of the conidiogenous cell and with successive conidia having alternate orientations with a zipper- or chevron-like arrangement resulting in flat, ribbon-like chains. 描述后生形态的 Entomopathogenic 属 Evlachovaea 与其他真菌不同，它的分生孢子斜向产孢细胞的轴，且连续的分生孢子以拉链或人字形排列交替取向，形成扁平的、带状的链。
Morphological and molecular studies of six Evlachovaea-like isolates baited from Central Brazilian soils using Triatoma infestans (a vector of Chagas disease) and of other entomopathogens with Evlachovaea-like conidiogenesis led to a re-evaluation of the status of this little known fungal genus. 利用中美洲巴西土壤中的三带喙库蚊（一种恰加斯病的传播媒介）诱饵和其他具有 Evlachovaea 样分生孢子发生的昆虫病原真菌，对 6 个 Evlachovaea 样分离物进行了形态和分子研究，导致对这个鲜为人知的真菌属的地位进行了重新评估。
The Brazilian isolates formed two distinct groups based on gene sequences for both the internal transcribed spacer (ITS) and translation elongation factor (EF-1α) genes, morphology, and growth patterns; both groups also differed from the type species, Evlachovaea kintrischica. 基于内部转录间隔区（ITS）和翻译延伸因子（EF-1α）基因、形态和生长模式的基因序列，巴西分离物形成了两个明显的组；这两个组也与模式种 Evlachovaea kintrischica 不同。
More detailed studies of these fungi indicated that the alternatingly oblique orientations of forming conidia are neither a stable nor invariant character (even on single phialides). 对这些真菌的更详细研究表明，形成分生孢子的交替斜向取向既不是稳定的，也不是不变的特征（即使在单个产孢细胞上也是如此）。
Furthermore, the molecular cladistic analysis unambiguously placed the Evlachovaea isolates firmly within the genus Isaria (Hypocreales: Cordycipitaceae). 此外，分子系统发育分析明确将 Evlachovaea 分离物置于 Isaria 属（Hypocreales：Cordycipitaceae）内。
The ITS sequences of E. kintrischica were very similar or even identical to those of Isaria amoenerosea and Isaria cateniobliqua, thereby suggesting that E. kintrischica is a synonym of one of these species, and that the genus Evlachovaea must be treated as a later synonym of Isaria, which must now be recognized to include several highly divergent modes of conidiogenesis. E. kintrischica 的 ITS 序列与 Isaria amoenerosea 和 Isaria cateniobliqua 的序列非常相似，甚至完全相同，这表明 E. kintrischica 是这些物种之一的同义词，而 Evlachovaea 属必须被视为 Isaria 的后同物异名，现在必须认识到 Isaria 包括几种高度分化的分生孢子发生模式。
These taxonomic findings are discussed in the context of dramatic changes recently imposed on the nomenclatural standards used to determine the correct names of all pleomorphic fungi. 这些分类学发现是在最近对用于确定所有多态真菌正确名称的命名标准进行的重大更改的背景下讨论的。
