Mongkolsamrit Suchada, Noisripoom Wasana, Thanakitpipattana Donnaya, Wutikhun Tuksadon, Spatafora Joseph W, Luangsa-Ard Jennifer
a Microbe Interaction and Ecology Laboratory, Biodiversity and Biotechnological Resource Research Unit (BBR), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , 113 Thailand Science Park Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120 , Thailand.
b Nano Characterization Laboratory, National Nanotechnology Center (NANOTEC) , 111 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120 , Thailand.
Mycologia. 2018 Jan-Feb;110(1):230-257. doi: 10.1080/00275514.2018.1446651.
A new genus and eight new species, all with isaria-like phialides, are described in Cordycipitaceae from Thailand. The new genus, Samsoniella, is segregated from Akanthomyces based on morphological and molecular evidence. Samsoniella differs from Akanthomyces in producing orange cylindrical to clavate stromata with superficial perithecia and orange conidiophores with isaria-like phialides and white to cream conidia. A new combination for CBS 240.32, originally identified as Paecilomyces farinosus (Isaria farinosa), and CBS 262.58, originally identified as Penicillium alboaurantium, respectively, is made in Samsoniella. Two new species, Samsoniella aurantia and S. inthanonensis, are described from lepidopteran larvae. Two new species of Cordyceps, C. blackwelliae and C. lepidopterorum, were also found on coleopteran and lepidopteran larvae. Both produce isaria-like morphs with globose phialides and attenuated long necks and white mycelium in culture. The authors established a sexual-asexual link for Cordyceps javanica (= Isaria javanica) on lepidopteran larvae. Four new species, Akanthomyces kanyawimiae, A. sulphureus, A. thailandicus, and A. waltergamsii, were pathogenic on spiders, with some strains of A. kanyawimiae also found on unidentified insect larvae. These four species of Akanthomyces occur on the underside of leaves and produce white to cream white powdery conidia, whereas S. aurantia and S. inthanonensis were found in leaf litter and produce bright orange stromata and synnemata with white conidia. Another new combination, Akanthomyces ryukyuensis, is proposed. Phylogenetic analyses based on a combined data set comprising the nuc rDNA region encompassing the internal transcribed spacers 1 and 2 along with the 5.8S rDNA (ITS), nuc 28S rDNA (28S), partial sequences of translation elongation factor 1-α gene (TEF1), and the genes for RNA polymerase II largest (RPB1) and second-largest (RPB2) subunits strongly support the delimitation of these new species of Cordyceps, Akanthomyces, and in a new genus Samsoniella in Cordycipitaceae.
在泰国的虫草科中描述了一个新属和八个新物种，所有物种均具有类棒束孢的瓶梗。基于形态学和分子证据，新属桑氏菌属（Samsoniella）从刺孢菌属（Akanthomyces）中分离出来。桑氏菌属与刺孢菌属的不同之处在于，它产生橙色圆柱形至棒状的子座，具表生的子囊壳，以及具类棒束孢瓶梗的橙色分生孢子梗和白色至奶油色的分生孢子。分别对最初鉴定为粉质拟青霉（粉棒束孢）的CBS 240.32和最初鉴定为橙黄青霉的CBS 262.58进行了桑氏菌属的新组合。从鳞翅目幼虫中描述了两个新物种，即橙色桑氏菌（Samsoniella aurantia）和茵他侬桑氏菌（S. inthanonensis）。还在鞘翅目和鳞翅目幼虫上发现了两种新的虫草，即布莱克韦尔虫草（Cordyceps blackwelliae）和鳞翅目虫草（C. lepidopterorum）。两者在培养物中均产生具有球形瓶梗和细长颈部的类棒束孢形态以及白色菌丝体。作者建立了鳞翅目幼虫上爪哇虫草（=爪哇棒束孢）性型与无性型之间的联系。四个新物种，即卡尼亚维米刺孢菌（Akanthomyces kanyawimiae）、硫黄刺孢菌（A. sulphureus）、泰国刺孢菌（A. thailandicus）和沃尔特甘姆斯刺孢菌（A. waltergamsii），对蜘蛛具有致病性，在未鉴定的昆虫幼虫上也发现了一些卡尼亚维米刺孢菌菌株。这四种刺孢菌属物种出现在叶片下表面，产生白色至奶油白色的粉状分生孢子，而橙色桑氏菌和茵他侬桑氏菌则在落叶层中发现，产生带有白色分生孢子亮橙色的子座和分生孢子梗束。提出了另一个新组合，琉球刺孢菌（Akanthomyces ryukyuensis）。基于包含核糖体DNA区域（包括内部转录间隔区1和2以及5.8S核糖体DNA（ITS））、核糖体28S DNA（28S）、翻译延伸因子1-α基因（TEF1）的部分序列以及RNA聚合酶II最大亚基（RPB1）和第二大亚基（RPB2）基因的组合数据集进行的系统发育分析，有力地支持了虫草科中这些新的虫草属、刺孢菌属以及新属桑氏菌属物种的界定。
