Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001 Heilongjiang, China.
Vet Microbiol. 2013 Apr 12;163(1-2):157-61. doi: 10.1016/j.vetmic.2012.11.032. Epub 2012 Nov 29.
Porcine parvovirus (PPV) is a major causative agent of reproductive failure in swine, which currently affects the swine industry worldwide. Although PPV was identified several years ago, little is known about how it overcomes host innate immunity. In this study, we used quantitative real-time PCR and a luciferase reporter assay to determine whether PPV infection induces type I interferon (IFN-α and IFN-β) and whether PPV infection blocks dsRNA-induced IFN-β promoter activation in cell cultures. The results indicate that PPV does not induce type I interferon and that the NS2 protein of PPV could blocks dsRNA-induced IFN-β promoter activation.
猪细小病毒（PPV）是一种主要的猪繁殖失败的病原体，目前影响着全球的养猪业。尽管 PPV 已经被发现了几年，但人们对它如何克服宿主先天免疫知之甚少。在这项研究中，我们使用定量实时 PCR 和荧光素酶报告基因检测来确定 PPV 感染是否诱导 I 型干扰素（IFN-α 和 IFN-β），以及 PPV 感染是否阻断细胞培养中 dsRNA 诱导的 IFN-β 启动子激活。结果表明，PPV 不诱导 I 型干扰素，而 PPV 的 NS2 蛋白可以阻断 dsRNA 诱导的 IFN-β 启动子激活。
