Department of Molecular Genetics and Cell Biology, Ulm University, Germany.
FEBS Lett. 2013 Feb 14;587(4):328-38. doi: 10.1016/j.febslet.2012.12.025. Epub 2013 Jan 16.
A proteomics screen was initiated to identify Rab proteins regulating transport to and away from peroxisomes. Mass spectrometry-based protein correlation profiling of rat liver organelles and immunofluorescence analysis of the peroxisome candidate Rab proteins revealed Rab6, Rab10, Rab14 and Rab18 to associate with the peroxisomal membrane. While Rab14 localized to peroxisomes predominantly in its dominant-active form, other Rab proteins associated with peroxisomes in both their GTP- and GDP-bound state. In summary, our data suggest that Rab6, Rab10, Rab14 and Rab18 associate with the peroxisomal compartment and similar as previously shown for Rab8, Rab18 in its GDP-bound state favors peroxisome proliferation.
启动蛋白质组学筛选以鉴定调节向和远离过氧化物酶体运输的 Rab 蛋白。大鼠肝细胞器的基于质谱的蛋白质相关分析和过氧化物酶体候选 Rab 蛋白的免疫荧光分析显示 Rab6、Rab10、Rab14 和 Rab18 与过氧化物酶体膜相关。虽然 Rab14 主要以其显性活性形式定位于过氧化物酶体，但其他 Rab 蛋白在其 GDP 和 GTP 结合状态下都与过氧化物酶体相关。总之，我们的数据表明 Rab6、Rab10、Rab14 和 Rab18 与过氧化物酶体隔室相关，类似于先前显示的 Rab8 和 Rab18 在其 GDP 结合状态下有利于过氧化物酶体增殖。
