National Reference Laboratory on Viral Hepatitis, Institute of Tropical Medicine Pedro Kourí (IPK), Autopista Novia del Mediodía Km 6 ½ e/Autopista Nacional y Carretera Central, La Lisa. P.O. Box: 601, Marianao 13, Havana City, Cuba.
Infect Genet Evol. 2013 Mar;14:335-9. doi: 10.1016/j.meegid.2012.12.022. Epub 2013 Jan 16.
This study was conduced to determinate the seroprevalence and risk factors associated to hepatitis E virus (HEV) exposition, in individuals who work in pig farms located at western of Artemise Province. The presence of HEV in human and swine samples and the phylogenetic analysis were evaluated. One hundred six workers (with an age range of 18-70years) were enrolled in this study. Two groups were defined, 69 employees with swine related occupations and 37 workers without contact with pigs. None had abroad travel history. Serum samples were tested for immunoglobulins (Ig) M, G and A against HEV. Individual fecal samples were obtained from 57 workers and 53 swine. All feces were tested for HEV RNA using reverse transcription PCR (RT-PCR). The amplification products were sequenced and phylogenetically analyzed by using MEGA5 software. A total of 38 (35.8%) (95% CI: 26.2-45.4) sera was positive for antibodies against HEV (anti-HEV). These were higher in persons who work in contact with swine compare as individuals with occupations without pig contact (40.5%, 28/69, 95% CI: 28.2-52.8, versus 27.0%, 10/37, 95% CI: 11.3-42.6, respectively). The prevalence of anti-HEV was higher in workers with an age range of 60-70years old and time-work 10-13years. HEV RNA was detected in 8 (14.0%) of 57 human fecal samples and 10 (18.8%) of 53 swine fecal samples. Phylogenetic analysis was performed on 7 amplification products obtained from 3 human and 4 swine fecal samples. Human and swine HEV sequences were closely related (94-99% nucleotide homology) and belonged to HEV genotype 3, subtype 3a.
这项研究旨在确定西部阿尔特米斯省养猪场工作人员的戊型肝炎病毒（HEV）血清流行率和相关危险因素。评估了人类和猪样本中 HEV 的存在以及系统进化分析。本研究纳入了 106 名（年龄 18-70 岁）工人。将这 106 名工人分为两组，一组是 69 名与猪有关的员工，另一组是 37 名没有与猪接触的员工。他们都没有出国旅行史。采集血清样本检测抗 HEV 的免疫球蛋白（Ig）M、G 和 A。从 57 名工人和 53 头猪中获得了单独的粪便样本。所有粪便均采用逆转录 PCR（RT-PCR）检测 HEV RNA。使用 MEGA5 软件对扩增产物进行测序和系统进化分析。共有 38 名（35.8%）（95%置信区间：26.2-45.4）血清对 HEV 抗体呈阳性（抗-HEV）。与无猪接触的员工相比，与猪接触的员工（40.5%，28/69，95%置信区间：28.2-52.8 与 27.0%，10/37，95%置信区间：11.3-42.6）更高。60-70 岁和工作时间 10-13 年的工人中，抗-HEV 的患病率更高。从 57 份人粪便样本和 53 份猪粪便样本中检测到 HEV RNA。从 3 个人类和 4 个猪粪便样本中获得的 7 个扩增产物进行了系统进化分析。人源性和猪源性 HEV 序列密切相关（94-99%核苷酸同源性），属于 HEV 基因型 3，亚型 3a。
