Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong 266101, PR China.
Bioresour Technol. 2013 Feb;130:731-8. doi: 10.1016/j.biortech.2012.11.116. Epub 2012 Dec 6.
To understand the mechanism of lipid accumulation and the corresponding metabolic changes of the microalga Nannochloropsis oceanica IMET1, the lipid content, fatty acid composition and metabolic profile were investigated via batch culture under nutrient deficiency and chemostatic culture under nitrate limitation. The results indicated that the triacylglycerol-neutral lipids were significantly accumulated through an acyl-CoA dependent pathway, while the polar lipids were partially converted to triacylglycerol through an acyl-CoA independent pathway. The fatty acid compositions of the polar lipids changed concurrently with the length of time of the nutrient deficiency, while the fatty acid compositions of the neutral lipids remained nearly consistent. The concentrations of several major osmolytes were significantly changed under chemostatic conditions with different nitrogen concentrations, which reflect the membrane property changes caused by the alteration of the polar lipid composition.
为了了解海洋微藻盐藻 Nannochloropsis oceanica IMET1 中脂质积累的机制和相应的代谢变化，通过在营养缺乏条件下进行分批培养和在硝酸盐限制条件下进行恒化培养，研究了其脂质含量、脂肪酸组成和代谢谱。结果表明，三酰基甘油-中性脂质通过酰基辅酶 A 依赖途径显著积累，而极性脂质通过酰基辅酶 A 非依赖途径部分转化为三酰基甘油。极性脂质的脂肪酸组成随着营养缺乏时间的延长而发生变化，而中性脂质的脂肪酸组成则基本保持不变。在不同氮浓度的恒化条件下，几种主要渗透物的浓度发生了显著变化，这反映了极性脂质组成改变引起的膜性质变化。
