不同氮供应条件下海洋微藻三角褐指藻中 TAG 和 EPA 积累的特点。
The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes.
机构信息
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Bioresour Technol. 2015 Mar;179:483-489. doi: 10.1016/j.biortech.2014.12.012. Epub 2014 Dec 11.
The strategy of nitrogen limitation has been widely applied to enhance lipid production in microalgae. The changes of cellular composition, and the characteristics of triacylglycerol (TAG) and eicosapentaenoic acid (EPA) accumulation in Nannochloropsis oceanica IMET1 were investigated. The results revealed that after nitrogen limitation TAG rather than carbohydrate was the dominant carbon sink in N. oceanica IMET1. Different nitrogen supplementation strategies were applied in order to achieve high TAG and EPA productivity, respectively. Limited nitrogen was supplied to improve TAG production, and a maximum productivity of 29.44 mg L(-1) d(-1) was obtained, which was a 6.74-fold increase compared to nitrogen-depleted cultivation. The highest EPA productivity of 7.66 mg L(-1) d(-1) was achieved under nitrogen-replete cultivation, which is different from the condition for TAG maximum productivity because the EPA is in glycolipids and phospholipids mainly. The fatty acid composition analysis identified the source of acyl group in TAG accumulation.
氮限制策略已被广泛应用于提高微藻的油脂产量。本研究考察了海洋盐藻（Nannochloropsis oceanica IMET1）细胞组成的变化，以及三酰基甘油（TAG）和二十碳五烯酸（EPA）积累的特点。结果表明，氮限制后，TAG 而非碳水化合物成为海洋盐藻 IMET1 的主要碳汇。采用不同的氮补充策略分别实现了高 TAG 和 EPA 生产力。有限的氮供应提高了 TAG 的产量，最大生产力达到 29.44mg/L/d，与氮饥饿培养相比增加了 6.74 倍。在氮充足的条件下，EPA 的最大生产力为 7.66mg/L/d，这与 TAG 最大生产力的条件不同，因为 EPA 主要存在于糖脂和磷脂中。脂肪酸组成分析确定了 TAG 积累中酰基来源。
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