School of Chemical Engineering, The University of Adelaide, Adelaide 5005, SA, Australia.
Colloids Surf B Biointerfaces. 2013 Apr 1;104:276-81. doi: 10.1016/j.colsurfb.2012.12.020. Epub 2012 Dec 29.
Lipid vesicles have received significant attention in areas ranging from pharmaceutical and biomedical engineering to novel materials and nanotechnology. Microfluidic-based synthesis of liposomes offers a number of advantages over the more traditional synthesis methods such as extrusion and sonication. One such microfluidic approach is microfluidic hydrodynamic focusing (MHF), which has been used to synthesize nanoparticles and vesicles of various lipids. We show here that this method can be utilized in synthesis of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles with controllable size. Since POPC is among the primary constituents of cellular membranes, this work is of direct applicability to modelling of biological systems and development of nano-containers with higher biologic compatibility for pharmaceutical and medical applications.
脂质体在从制药和生物医学工程到新型材料和纳米技术等领域受到了广泛关注。基于微流控的脂质体合成相对于挤出和超声等更传统的合成方法具有许多优势。一种这样的微流控方法是微流控流体动力学聚焦（MHF），它已被用于合成各种脂质的纳米颗粒和囊泡。我们在这里展示了该方法可用于合成具有可控尺寸的 1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）囊泡。由于 POPC 是细胞膜的主要成分之一，因此这项工作可直接应用于生物系统建模以及开发具有更高生物相容性的纳米容器，用于制药和医疗应用。
