Department of Burns and Plastic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi-an 710038, China.
Acta Pharmacol Sin. 2013 Mar;34(3):367-72. doi: 10.1038/aps.2012.159. Epub 2013 Jan 21.
To examine the changes in electrolyte concentrations after addition of zeolite-based hemostat QuikClot in blood and the effects of zeolite on blood coagulation in vitro.
Fresh blood was taken from healthy adult volunteers and sheep, and the electrolyte concentrations in blood were measured using a blood electrolyte analyzer. Zeolite Saline Solution (ZSS) was prepared by addition of 2 g zeolite to 0.9% NaCl solution (4, 8, or 16 mL). The electrolytes in ZSS were measured using inductively coupled plasma atomic emission spectroscopy. The prothrombin time (PT) and activated partial thromboplastin time (APTT) of blood were measured using the test tube method. The activated clotting time (ACT) and clotting rate (CR) of blood were measured with Sonoclot Coagulation and Platelet Function Analyzer.
Addition of zeolite (50 and 100 mg) in 2 mL human blood significantly increased Ca(2+) concentration, while Na(+) and K(+) concentrations were significantly decreased. Addition of zeolite (50 and 100 mg) in 0.9% NaCl solution (2 mL) caused similar changes in Ca(2+) and Na(+) concentrations. Si(4+) (0.2434 g/L) and Al(3+) (0.2575 g/L) were detected in ZSS (2 g/8 mL). Addition of ZSS in sheep blood shortened APTT in a concentration dependent manner, without changing PT. ZSS or aqueous solution of CaCl2 that contained Ca(2+) concentration identical to that of ZSS significantly shortened ACT in human blood without significantly changing CR, and the effect of ZSS on ACT was not significantly different from that of CaCl2.
Zeolite releases Ca(2+) into blood, thus accelerating the intrinsic pathway of blood coagulation and shortening the clot formation time.
研究沸石基止血剂 QuikClot 加入血液后电解质浓度的变化，以及沸石对体外血液凝血的影响。
从健康成年志愿者和绵羊中抽取新鲜血液，使用血液电解质分析仪测量血液中的电解质浓度。通过向 0.9%NaCl 溶液（4、8 或 16mL）中添加 2g 沸石来制备沸石生理盐水溶液（ZSS）。使用电感耦合等离子体原子发射光谱法测量 ZSS 中的电解质。使用试管法测量血液的凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）。使用 Sonoclot 凝血和血小板功能分析仪测量血液的活化凝血时间（ACT）和凝血速率（CR）。
在 2mL 人血中添加 50 和 100mg 沸石显著增加了 Ca(2+)浓度，而 Na(+)和 K(+)浓度显著降低。在 0.9%NaCl 溶液（2mL）中添加 50 和 100mg 沸石也导致 Ca(2+)和 Na(+)浓度发生类似变化。在 ZSS（2g/8mL）中检测到 Si(4+)（0.2434g/L）和 Al(3+)（0.2575g/L）。在绵羊血液中，ZSS 以浓度依赖的方式缩短 APTT，而不改变 PT。ZSS 或含有与 ZSS 相同 Ca(2+)浓度的 CaCl2 水溶液显著缩短了人血中的 ACT，而对 CR 没有显著影响，并且 ZSS 对 ACT 的影响与 CaCl2 没有显著差异。
沸石将 Ca(2+)释放到血液中，从而加速血液凝血的内源性途径，缩短血栓形成时间。
