Zhong Bo-xiong, Zhang Wan, Liu Wei-zhi, Pang Zong-ran, Cui Jian
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.
Zhong Yao Cai. 2011 Jun;34(6):872-6.
To observe the effects of raw and charred Agi on hemostasis and its mechanism.
The rabbit bleeding time was measured by traumatic hemorrhage test, and the clotting time was measured by tube test. The rabbit prothrombin time (PT), activated partial thormboplastia time (APTT), thrombin time (TT), fibrinogen (FIB), plasma recalcification time (PRT), euglobulin lysis time (ELT), max platelet aggregation rate (MPAR) were measured by solidification method, turbidimetry and tube test to analyze the effects of raw and charred Agi on rabbit coagulation-fibrinolysis system and platelet function.
The medium doses and high doses of raw Agi groups, all of the groups of charred Agi decreased rabbit BT obviously (P<0.01 or P<0.05); all of the groups of raw and charred Agi declined rabbits CT (P<0.01 or P<0.05). All of the doses groups of raw and charred Agi had no apparent influences on PT (P>0.05); the high dose of raw Agi group and all of the groups of charred Agi decreased APTT apparently (P<0.01 or P<0.05) and prolonged ELT (P<0.01 or P<0.05); the high doses groups of raw and charred Agi decreased TT apparently (P<0.01 or P<0.05); the medium and high doses groups of charred Agi increased FIB obviously (P<0.01 or P<0.05); the high doses group of charred Agi showed the decreased PRT significantly (P<0.05) and increased MPAR obviously (P<0.01).
Raw Agi can play its role in hemostasis and coagulation by affecting the intrinsic pathway of coagulation and fibrinolytic system. These effects are inhanced after processing drugs; moreover, the charred Agi could increase FIB and MPAR with promoting more in blood coagulation.
观察生、炒艾叶对止血作用的影响及其机制。
采用创伤性出血法测定家兔出血时间，试管法测定凝血时间。采用凝固法、比浊法和试管法测定家兔凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、凝血酶时间（TT）、纤维蛋白原（FIB）、血浆复钙时间（PRT）、优球蛋白溶解时间（ELT）、最大血小板聚集率（MPAR），分析生、炒艾叶对家兔凝血-纤溶系统及血小板功能的影响。
生艾叶中、高剂量组，炒艾叶各剂量组均能明显缩短家兔出血时间（P<0.01或P<0.05）；生、炒艾叶各剂量组均能缩短家兔凝血时间（P<0.01或P<0.05）。生、炒艾叶各剂量组对PT均无明显影响（P>0.05）；生艾叶高剂量组和炒艾叶各剂量组均能明显缩短APTT（P<0.01或P<0.05），延长ELT（P<0.01或P<0.05）；生、炒艾叶高剂量组均能明显缩短TT（P<0.01或P<0.05）；炒艾叶中、高剂量组能明显升高FIB（P<0.01或P<0.05）；炒艾叶高剂量组能明显缩短PRT（P<0.05），明显升高MPAR（P<0.01）。
生艾叶可通过影响内源性凝血途径及纤溶系统发挥止血凝血作用。炮制后该作用增强；此外，炒艾叶可升高FIB和MPAR，促进血液凝固作用更强。
