Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Dalton Trans. 2013 Mar 21;42(11):3957-67. doi: 10.1039/c2dt32114a. Epub 2013 Jan 21.
Four metal complexes [Pd(L(1))Cl(2)·2H(2)O] (1), [Pt(L(1))Cl(2)·2H(2)O] (2), [Pd(L(2))Cl(2)·H(2)O] (3) and [Pt(L(2))Cl(2)·H(2)O] (4) (L(1) = 2,2'-bipyridyl-5,5'-dicarboxylic acid, L(2) = 2,2'-bipyridyl-4,4'-dicarboxylic acid) have been synthesized under hydrothermal conditions and fully characterized by IR and (1)H-NMR spectra, elemental analysis, and X-ray single crystal diffraction analysis. Interactions of these complexes with fish sperm DNA (FS-DNA) were investigated using UV-Vis absorption and fluorescence spectroscopic methods. Further insight was brought by quantum chemistry calculations by means of G03 package and taking B3LYP functional Lanl2dz Gen basis set. Agarose gel electrophoresis run on pBR322 plasmid DNA gave proof that all four complexes exhibit efficient DNA cleavage. Complexes 1-4 manifested cytotoxic specificity and a significant cancer cell inhibitory rate. Independent apoptosis tests under the light microscope, performed on hematoxylin-eosin stained HeLa cells, evidenced morphological changes induced by all the complexes.
四种金属配合物[Pd(L(1))Cl(2)·2H(2)O]（1）、[Pt(L(1))Cl(2)·2H(2)O]（2）、[Pd(L(2))Cl(2)·H(2)O]（3）和[Pt(L(2))Cl(2)·H(2)O]（4）（L(1) = 2,2'-联吡啶-5,5'-二羧酸，L(2) = 2,2'-联吡啶-4,4'-二羧酸）已在水热条件下合成，并通过红外光谱和（1）H-NMR 光谱、元素分析和 X 射线单晶衍射分析进行了充分的表征。使用紫外-可见吸收和荧光光谱法研究了这些配合物与鱼精 DNA（FS-DNA）的相互作用。进一步通过 G03 程序包和 Lanl2dz Gen 基本组，采用 B3LYP 函数进行量子化学计算，提供了更深入的见解。琼脂糖凝胶电泳在 pBR322 质粒 DNA 上进行，证明所有四个配合物都具有有效的 DNA 切割活性。配合物 1-4 表现出细胞毒性特异性和显著的癌细胞抑制率。在苏木精-伊红染色的 HeLa 细胞上进行的独立凋亡试验，在光镜下证明了所有配合物诱导的形态变化。
