Paradisi L, Negro F, Panagini C, Torrielli M V
Boll Soc Ital Biol Sper. 1979 Sep 30;55(18):1877-83.
G-6-Pase activity was investigated in the microsomal fraction from rat liver in the presence of carbon tetrachloride and/or propyl gallate (PG), reduced glutathione (GSH) and superoxide dismutase. Results obtained "in vitro" demonstrated that CCl4 induced a 60% inhibition of the microsomal enzyme activity. Moreover, a marked inhibition of G-6-Pase activity was found also when propyl gallate and reduced glutathione were added, at different concentrations, to incubation mixture. In addition, these drugs were unable to interfere with the dangerous effect exerted on the enzymatic activity by the haloalkane. Additional experiments carried out "in vivo" with propyl gallate produced evidence that intraperitoneal administration of the antioxidant was followed by a significant inhibition of G-6-Pase activity, while the damaging action of CCl4 was unaffected. Some possible explanations of these results are reported.
在存在四氯化碳和/或没食子酸丙酯（PG）、还原型谷胱甘肽（GSH）和超氧化物歧化酶的情况下，对大鼠肝脏微粒体部分的葡萄糖-6-磷酸酶（G-6-Pase）活性进行了研究。“体外”实验结果表明，四氯化碳可使微粒体酶活性受到60%的抑制。此外，当向孵育混合物中添加不同浓度的没食子酸丙酯和还原型谷胱甘肽时，也发现葡萄糖-6-磷酸酶活性受到显著抑制。此外，这些药物无法干扰卤代烷对酶活性产生的有害作用。用没食子酸丙酯进行的“体内”额外实验表明，腹腔注射抗氧化剂后，葡萄糖-6-磷酸酶活性受到显著抑制，而四氯化碳的损伤作用未受影响。文中报道了对这些结果的一些可能解释。
