Bragt P C, Bansberg J I, Bonta I L
Agents Actions Suppl. 1980;7:214-9.
Changes in hepatic oxidative events have been studied during extrahepatic granulomatous inflammation in the rat, together with the local anti-inflammatory effects of scavengers of reactive oxygen species. During remote localized inflammation in the rat, we observed increased hepatic lipid peroxidation and reduced hepatic levels of protecting substances such as ascorbic acid, catalase and reduced glutathione, the specific substrate for the peroxide-metabolizing enzyme, glutathione peroxidase. The levels of superoxide dismutase did not alter significantly during the period of investigation (7 days). The half-life of aminopyrine was longer in rats with a stronger inflammatory response. In addition, catalase and the anti-oxidants, alpha-tocopherol and propyl gallate, inhibited granuloma development on local injection during the acute phase inflammation. Superoxide dismutase alone was devoid of anti-inflammatory effects, but markedly enhanced the effect of catalase. Scavengers of hydroxyl radicals and of singlet oxygen failed to display anti-inflammatory activity. The results indicate that peroxides may act as mediators of inflammation and that increased lipid peroxidation is not limited to the site of inflammation.
在大鼠肝外肉芽肿性炎症期间，研究了肝脏氧化事件的变化，以及活性氧清除剂的局部抗炎作用。在大鼠的远隔局部炎症期间，我们观察到肝脏脂质过氧化增加，以及肝脏中诸如抗坏血酸、过氧化氢酶和还原型谷胱甘肽等保护物质的水平降低，还原型谷胱甘肽是过氧化物代谢酶谷胱甘肽过氧化物酶的特定底物。在研究期间（7天），超氧化物歧化酶的水平没有显著改变。在炎症反应较强的大鼠中，氨基比林的半衰期更长。此外，过氧化氢酶以及抗氧化剂α-生育酚和没食子酸丙酯在急性期炎症期间局部注射时可抑制肉芽肿形成。单独的超氧化物歧化酶没有抗炎作用，但能显著增强过氧化氢酶的作用。羟自由基和单线态氧的清除剂没有显示出抗炎活性。结果表明，过氧化物可能作为炎症介质起作用，并且脂质过氧化增加并不局限于炎症部位。
