Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
J Hazard Mater. 2013 Feb 15;246-247:319-23. doi: 10.1016/j.jhazmat.2012.12.025. Epub 2012 Dec 20.
In order to evaluate the effect of anaerobic digestion intermediates and antibiotics in pharmaceutical wastewaters on anaerobic digestion process, their acute toxicities were tested using the 15 min median inhibitory concentration (IC(50)) at pH 7.00 ± 0.05. The results showed that the IC(50) of ethanol, acetate, propionate and butyrate were 19.40, 20.71, 10.47 and 12.17 g L(-1) respectively, which suggested the toxicity descended in the order of propionate, butyrate, ethanol and acetate. The IC(50) of aureomycin, polymyxin and chloromycetin were 12.06, 6.24 and 429.90 mg L(-1) respectively, which indicated the toxicity descended in the order of polymyxin, aureomycin and chloromycetin. Using equitoxic ratio mixing method, the joint toxicities of five groups referred by A (four anaerobic digestion intermediates), B (four anaerobic digestion intermediates and aureomycin), C (four anaerobic digestion intermediates and polymyxin), D (four anaerobic digestion intermediates and chloromycetin) and E (four anaerobic digestion intermediates, aureomycin, polymyxin and chloromycetin) were investigated respectively. Their interactions were additive (A), synergistic (B), additive (C), synergistic (D) and synergistic (E). The investigation would lay a basis for the optimization of anaerobic biotechnology for pharmaceutical wastewater treatment.
为了评估制药废水中的厌氧消化中间产物和抗生素对厌氧消化过程的影响，采用 pH 值为 7.00±0.05 时的 15 分钟中值抑制浓度（IC50）测试了它们的急性毒性。结果表明，乙醇、乙酸、丙酸和丁酸的 IC50 分别为 19.40、20.71、10.47 和 12.17gL-1，表明毒性按丙酸、丁酸、乙醇和乙酸的顺序降低。金霉素、多粘菌素和氯霉素的 IC50 分别为 12.06、6.24 和 429.90mgL-1，表明毒性按多粘菌素、金霉素和氯霉素的顺序降低。采用等毒性比混合法，分别研究了由 A（四种厌氧消化中间产物）、B（四种厌氧消化中间产物和金霉素）、C（四种厌氧消化中间产物和多粘菌素）、D（四种厌氧消化中间产物和氯霉素）和 E（四种厌氧消化中间产物、金霉素、多粘菌素和氯霉素）五个组别的联合毒性。它们的相互作用分别为相加（A）、协同（B）、相加（C）、协同（D）和协同（E）。该研究为优化制药废水的厌氧生物技术奠定了基础。
