Ji Jun-Yuan, Xing Ya-Juan, Zheng Ping
Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
Huan Jing Ke Xue. 2012 Dec;33(12):4367-72.
In order to determine the toxicity of antibiotics and anaerobic digestion intermediates on anaerobic treatment of pharmaceutical wastewaters containing antibiotics, the single and joint toxicities of some antibiotics and intermediates to Photobacterium phosphoreum were tested by using the 15-min half inhibitory concentration (15 min-IC50) at pH = 7.00 +/- 0.05. The results showed that the 15 min-IC50 of ethanol, acetate, propionate and butyrate were 19.40, 20.71, 10.47 and 12.17 g x L(-1), respectively, which indicated that the toxicity descended in the order of propionate, butyrate, ethanol and acetate. The 15 min-IC50 of Amoxicillin, Kanamycin, Lincomycin and Ciprofloxacin were 3.99, 5.11, 4.32 and 5.63 g x L(-1), respectively, so the toxicity descended in the order of Amoxicillin, Lincomycin, Kanamycin and Ciprofloxacin. Using equal effect mixing method, the joint toxicity of four anaerobic digestion intermediates, the four intermediates together with Amoxicillin, Ciprofloxacin, Kanamycin, Lincomycin individually and all together were investigated, which demonstrated that the first three interactions were additive and the last three were synergistic. The observations have laid a foundation for control and optimization of anaerobic biotechnology for pharmaceutical wastewater containing antibiotics.
为了确定抗生素和厌氧消化中间产物对含抗生素制药废水厌氧处理的毒性，在pH = 7.00±0.05条件下，采用15分钟半数抑制浓度（15 min-IC50）测试了某些抗生素和中间产物对费氏弧菌的单一毒性和联合毒性。结果表明，乙醇、乙酸、丙酸和丁酸的15 min-IC50分别为19.40、20.71、10.47和12.17 g·L⁻¹，这表明毒性顺序为丙酸、丁酸、乙醇和乙酸。阿莫西林、卡那霉素、林可霉素和环丙沙星的15 min-IC50分别为3.99、5.11、4.32和5.63 g·L⁻¹，所以毒性顺序为阿莫西林、林可霉素、卡那霉素和环丙沙星。采用等效混合法，研究了四种厌氧消化中间产物、这四种中间产物分别与阿莫西林、环丙沙星、卡那霉素、林可霉素以及它们全部一起的联合毒性，结果表明前三种相互作用为相加作用，后三种为协同作用。这些观察结果为含抗生素制药废水厌氧生物技术的控制和优化奠定了基础。
