Wilson A D, Clarke C J, Stokes C R
Department of Veterinary Medicine, University of Bristol, UK.
Scand J Immunol. 1990 Apr;31(4):443-51. doi: 10.1111/j.1365-3083.1990.tb02791.x.
Cholera toxin (CT) is a potent stimulator of IgA responses when administered orally and has been shown to promote IgA responses to a second protein such as keyhole limpet haemocyanin (KLH) if this is fed simultaneously. In this paper we show that whilst feeding 5 mg KLH with either 0.5 micrograms CT or 10 micrograms B subunit fails to stimulate a mucosal IgA response to KLH, feeding 0.5 microgram CT and 10 micrograms B subunit together with 5 mg KLH produces a local IgA anti-KLH response as great as that produced by 10 micrograms of whole CT. In addition to stimulating IgA responses in the lamina propria, preliminary results indicate that cellular responses are also stimulated, as we have demonstrated KLH antigen-driven proliferation of cells isolated from groups of mice fed either 10 micrograms CT + 5 mg KLH or 0.5 micrograms CT + 10 micrograms CTB + 5 mg KLH but not mice fed KLH alone or with either 10 micrograms CTB or 0.5 micrograms CT. These results indicate that the mucosal adjuvant action of CT is due to a synergistic effect involving both the GM1 binding of the B subunit and adenylate cyclase activation by the A subunit.
霍乱毒素（CT）口服时是IgA反应的强效刺激剂，并且已表明，如果同时喂食匙孔血蓝蛋白（KLH）等第二种蛋白质，它能促进对该蛋白质的IgA反应。在本文中，我们表明，虽然将5毫克KLH与0.5微克CT或10微克B亚基一起喂食不能刺激对KLH的黏膜IgA反应，但将0.5微克CT和10微克B亚基与5毫克KLH一起喂食会产生与10微克完整CT产生的局部IgA抗KLH反应一样强烈的反应。除了刺激固有层中的IgA反应外，初步结果表明细胞反应也受到刺激，因为我们已经证明，从喂食10微克CT + 5毫克KLH或0.5微克CT + 10微克CTB + 5毫克KLH的小鼠组中分离出的细胞会出现KLH抗原驱动的增殖，而单独喂食KLH或与10微克CTB或0.5微克CT一起喂食的小鼠则不会。这些结果表明，CT的黏膜佐剂作用是由于一种协同效应，该效应涉及B亚基的GM1结合和A亚基对腺苷酸环化酶的激活。
